Yarrowia lipolytica has fast become a biotechnologically significant yeast for its ability to
accumulate lipids to high levels. While there exists a suite of synthetic biology tools for …

Use of the CRISPR/Cas9 RNA-guided endonuclease complex has recently enabled the
generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we …

The ability to add, remove, or change DNA sequences is essential to studies that investigate
the genetic underpinning of phenotypic traits. With its unprecedented efficiency and stunning …

Genome editing, which involves the precise manipulation of cellular DNA sequences to alter
cell fates and organism traits, has the potential to both improve our understanding of human …

Highlights•Presented the typical workflow of a CRISPR screen in plants.•Collected and
described appropriate genome editors for large-scale editing in plant.•Envisioned a virus …

CRISPR-Cas9 technologies have dramatically increased the ease of targeting DNA
sequences in the genomes of living systems. The fusion of chromatin-modifying domains to …

Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces
cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module …

The availability of a near-complete (96%) collection of gene-deletion mutants in
Saccharomyces cerevisiae greatly facilitates the systematic analyses of gene function in …

A genome-wide deletion library is a powerful tool for probing gene functions and one has
recently become available for the fission yeast Schizosaccharomyces pombe. Here we use …

Yeast is a powerful model for systems genetics. We present a versatile, time-and labor-
efficient method to functionally explore the Saccharomyces cerevisiae genome using …