Guide RNA Engineering Enables Dual Purpose CRISPR-Cpf1 for Simultaneous Gene Editing and Gene Regulation in Yarrowia lipolytica
A Ramesh, T Ong, JA Garcia, J Adams… - ACS Synthetic …, 2020 - ACS Publications
Yarrowia lipolytica has fast become a biotechnologically significant yeast for its ability to
accumulate lipids to high levels. While there exists a suite of synthetic biology tools for …
accumulate lipids to high levels. While there exists a suite of synthetic biology tools for …
Efficient Genome Editing in Caenorhabditis elegans with a Toolkit of Dual-Marker Selection Cassettes
Use of the CRISPR/Cas9 RNA-guided endonuclease complex has recently enabled the
generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we …
generation of double-strand breaks virtually anywhere in the C. elegans genome. Here, we …
Cas9 targeting and the CRISPR revolution
R Barrangou - Science, 2014 - science.org
The ability to add, remove, or change DNA sequences is essential to studies that investigate
the genetic underpinning of phenotypic traits. With its unprecedented efficiency and stunning …
the genetic underpinning of phenotypic traits. With its unprecedented efficiency and stunning …
The promise and challenge of therapeutic genome editing
JA Doudna - Nature, 2020 - nature.com
Genome editing, which involves the precise manipulation of cellular DNA sequences to alter
cell fates and organism traits, has the potential to both improve our understanding of human …
cell fates and organism traits, has the potential to both improve our understanding of human …
Large-scale genome editing in plants: approaches, applications, and future perspectives
T Liu, X Zhang, K Li, Q Yao, D Zhong, Q Deng… - Current Opinion in …, 2023 - Elsevier
Highlights•Presented the typical workflow of a CRISPR screen in plants.•Collected and
described appropriate genome editors for large-scale editing in plant.•Envisioned a virus …
described appropriate genome editors for large-scale editing in plant.•Envisioned a virus …
Transgenic mice for in vivo epigenome editing with CRISPR-based systems
MP Gemberling, K Siklenka, E Rodriguez… - Nature …, 2021 - nature.com
CRISPR-Cas9 technologies have dramatically increased the ease of targeting DNA
sequences in the genomes of living systems. The fusion of chromatin-modifying domains to …
sequences in the genomes of living systems. The fusion of chromatin-modifying domains to …
Genome-wide C-SWAT library for high-throughput yeast genome tagging
Here we describe a C-SWAT library for high-throughput tagging of Saccharomyces
cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module …
cerevisiae open reading frames (ORFs). In 5,661 strains, we inserted an acceptor module …
Genome-wide analysis of barcoded Saccharomyces cerevisiae gene-deletion mutants in pooled cultures
The availability of a near-complete (96%) collection of gene-deletion mutants in
Saccharomyces cerevisiae greatly facilitates the systematic analyses of gene function in …
Saccharomyces cerevisiae greatly facilitates the systematic analyses of gene function in …
Global fitness profiling of fission yeast deletion strains by barcode sequencing
A genome-wide deletion library is a powerful tool for probing gene functions and one has
recently become available for the fission yeast Schizosaccharomyces pombe. Here we use …
recently become available for the fission yeast Schizosaccharomyces pombe. Here we use …
Functional mapping of yeast genomes by saturated transposition
Yeast is a powerful model for systems genetics. We present a versatile, time-and labor-
efficient method to functionally explore the Saccharomyces cerevisiae genome using …
efficient method to functionally explore the Saccharomyces cerevisiae genome using …