The present studies investigated the subcellular distribution of acetylcholine's effects upon
the phosphorylation of tyrosine hydroxylase in isolated purified bovine adrenal chromaffin …

We have identified a 56‐kilodalton protein in cultured bovine adrenal chromaffin cells that is
phos‐phorylated when catecholamine secretion is stimulated. Immunodetection on Western …

Acetylcholine, the natural secretagogue, increased catecholamine biosynthesis in isolated
purified bovine adrenal chromaffin cells. This effect was calcium dependent, mimicked by …

Tryptic peptide fragments of tyrosine hydroxylase isolated from 32PO4-prelabeled bovine
adrenal chromaffin cells are resolved into seven phosphopeptides by reverse phase-high …

Antibodies raised to phosphorylated forms of tyrosine hydroxylase, the first and rate-limiting
enzyme in the catecholamine biosynthesis, were applied in immunohistochemical studies …

Under phosphorylating conditions, addition of Ca2+ or cyclic AMP to the 100,000 g
supernatant of purified bovine adrenal chromaffin cells increases both the incorporation of …

A method for simultaneous measurement of tyrosine hydroxylase (TH) activation and
phosphorylation in permeabilised and intact bovine adrenal chromaffin cells (BACCs) was …

The protein kinases and protein phosphatases that act on tyrosine hydroxylase in vivo have
not been established. Bovine adrenal chromaffin cells were permeabilized with digitonin …

Intact bovine adrenal medullary chromaffin cells were preincubated with 32 PO 4, and the
multiplesite phosphorylation of tyrosine hydroxylase (TH) was studied. Up to eight 32 P …

A monospecific antibody against tyrosine hydroxylase (TH), purified from a transplantable rat
pheochromocytoma, was produced in rabbits. Immunohistochemical techniques were …