Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA
RD Allen, PE Pellett, JA Stewart… - Journal of clinical …, 1995 - Am Soc Microbiol
We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus
(HCMV). The assay includes primer and probe sequences derived from conserved HCMV …
(HCMV). The assay includes primer and probe sequences derived from conserved HCMV …
Polymerase chain reaction assay for detection of human cytomegalovirus
DM Olive, M Simsek, S Al-Mufti - Journal of Clinical Microbiology, 1989 - Am Soc Microbiol
Direct detection of human cytomegalovirus (HCMV) from clinical specimens was examined
by using the polymerase chain reaction (PCR) for amplifying HCMV DNA. The efficiency of …
by using the polymerase chain reaction (PCR) for amplifying HCMV DNA. The efficiency of …
Enzymatic amplification of human cytomegalovirus sequences by polymerase chain reaction
K Hsia, DH Spector, J Lawrie… - Journal of Clinical …, 1989 - Am Soc Microbiol
Polymerase chain reaction (PCR) amplification was used to detect human cytomegalovirus
(HCMV) sequences. The fragments selected for amplification were fragments of 130 and …
(HCMV) sequences. The fragments selected for amplification were fragments of 130 and …
Cytomegalovirus DNA detection in sera from patients with active cytomegalovirus infections
M Brytting, W Xu, B Wahren… - Journal of clinical …, 1992 - Am Soc Microbiol
Using a specific and sensitive polymerase chain reaction method, we detected reliably the
presence of human cytomegalovirus (HCMV) DNA directly in serum samples collected at an …
presence of human cytomegalovirus (HCMV) DNA directly in serum samples collected at an …
Identification of human cytomegalovirus isolates by the polymerase chain reaction.
D Zipeto, E Silini, M Parea, E Percivalle… - Microbiologica, 1990 - europepmc.org
Fifty human cytomegalovirus (HCMV) isolates were recovered from different clinical
specimens (buffy coat, throat washing and urine) obtained from fifty patients (23 AIDS …
specimens (buffy coat, throat washing and urine) obtained from fifty patients (23 AIDS …
Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples …
D Zipeto, MG Revello, E Silini, M Parea… - Journal of Clinical …, 1992 - Am Soc Microbiol
The presence of human cytomegalovirus (HCMV) DNA in blood was investigated by the
polymerase chain reaction (PCR) in 293 blood samples from 86 immunocompromised …
polymerase chain reaction (PCR) in 293 blood samples from 86 immunocompromised …
Polymerase chain reaction detection of human cytomegalovirus in over 2000 blood specimens correlated with virus isolation and related to urinary virus excretion
E Drouet, S Michelson, G Denoyel… - Journal of virological …, 1993 - Elsevier
The polymerase chain reaction (PCR) as applied to human cytomegalovirus (HCMV)
detection should provide a valuable tool for rapid, reliable diagnosis of infection, thereby …
detection should provide a valuable tool for rapid, reliable diagnosis of infection, thereby …
Detection of human cytomegalovirus in peripheral mononuclear cells and urine samples using PCR
P Stanier, AD Kitchen, DL Taylor, AS Tyms - Molecular and cellular probes, 1992 - Elsevier
Samples of peripheral blood lymphocytes from 105 different blood donors were investigated
for the presence of human cytomegalovirus (HCMV) DNA using the polymerase chain …
for the presence of human cytomegalovirus (HCMV) DNA using the polymerase chain …
Highly sensitive single-step PCR protocol for diagnosis and monitoring of human cytomegalovirus infection in renal transplant recipients
OL Caballero, CL Menezes, MC Costa… - Journal of Clinical …, 1997 - Am Soc Microbiol
A multiplex, single-step PCR protocol for the detection of human cytomegalovirus (HCMV)
DNA is described. The protocol amplifies regions of the viral LA and IE genes and employs …
DNA is described. The protocol amplifies regions of the viral LA and IE genes and employs …
Simplified PCR set-up using a frozen preformulated mix for the detection of cytomegalovirus
B Kofler, A Klausegger - Diagnostic microbiology and infectious disease, 1999 - Elsevier
A simple PCR set-up for the detection of cytomegalovirus in clinical specimen was
developed. All components of the PCR master mix including Taq DNA polymerase, uracil N …
developed. All components of the PCR master mix including Taq DNA polymerase, uracil N …