We present a simple wide-field imaging technique, called HiLo microscopy, that is capable
of producing optically sectioned images in real time, comparable in quality to confocal laser …

It is well known that light-sheet illumination can enable optically sectioned wide-field
imaging of macroscopic samples. However, the optical sectioning capacity of a light-sheet …

High-throughput optical imaging is critical to obtain large-scale neural connectivity
information of brain in neuroscience. Using a digital mirror device and a scientific …

Electrically tunable lenses exhibit strong potential for fast motion-free axial scanning in a
variety of microscopes. However, they also lead to a degradation of the achievable …

We present an endomicroscope apparatus that exhibits out-of-focus background rejection
based on wide-field illumination through a flexible imaging fiber bundle. Our technique …

We present a simple modification to a conventional wide-field fluorescence microscope that
provides depth discrimination in thick tissues. The technique consists of illuminating a …

We describe a method of obtaining optical sectioning with a standard wide-field
fluorescence microscope. The method involves acquiring two images, one with nonuniform …

Large, living biological specimens present challenges to existing optical imaging techniques
because of their absorptive and scattering properties. We developed selective plane …

Optical sectioning microscopy is usually performed by means of a scanning, multi-shot
procedure in combination with non-uniform illumination. In this paper, we change the …

This Letter presents the first demonstration of multi-tile stitching for large scale 3D imaging in
single objective light-sheet microscopy. We show undistorted 3D imaging spanning …