Preparing individual Drosophila egg chambers for live imaging

TT Weil, RM Parton, I Davis - JoVE (Journal of Visualized Experiments), 2012 - jove.com
Live cell imaging is an important technique applied to a number of Drosophila tissues used
as models to investigate topics such as axis specification, cell differentiation and …

Isolation of Drosophila egg chambers for imaging

RM Parton, AM Vallés, IM Dobbie… - Cold Spring Harbor …, 2010 - cshprotocols.cshlp.org
The fruit fly Drosophila melanogaster is an important model for basic research into the
molecular mechanisms underlying cell function and development, as well as a major …

Cultivation and Live Imaging of Drosophila Ovaries

M Cetera, L Lewellyn, S Horne-Badovinac - Drosophila: Methods and …, 2016 - Springer
Drosophila egg chamber development depends on a number of dynamic cellular processes
that contribute to the final shape and function of the egg. We can gain insight into the …

A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers During Drug Treatments

AL Zajac, AM Williams, S Horne-Badovinac - … Oogenesis: Methods and …, 2023 - Springer
The Drosophila egg chamber is a powerful system to study epithelial cell collective migration
and polarized basement membrane secretion. A strength of this system is the ability to …

In Vitro Culturing and Live Imaging of Drosophila Egg Chambers: A History and Adaptable Method

NC Peters, CA Berg - Oogenesis: Methods and Protocols, 2016 - Springer
The development of the Drosophila egg chamber encompasses a myriad of diverse
germline and somatic events, and as such, the egg chamber has become a widely used and …

Culturing Drosophila Egg Chambers and Investigating Developmental Processes Through Live Imaging

L Manning, M Starz-Gaiano - Drosophila Oogenesis: Methods and …, 2015 - Springer
Drosophila oogenesis provides many examples of essential processes in development. A
myriad of genetic tools combined with recent advances in culturing egg chambers ex vivo …

Shining light on Drosophila oogenesis: live imaging of egg development

L He, X Wang, DJ Montell - Current opinion in genetics & development, 2011 - Elsevier
Drosophila oogenesis is a powerful model for the study of numerous questions in cell and
developmental biology. In addition to its longstanding value as a genetically tractable model …

Upright imaging of Drosophila embryos

M Belu, M Javier, K Ayasoufi, S Frischmann… - JoVE (Journal of …, 2010 - jove.com
Several well-known morphogenetic gradients and cellular movements occur along the
dorsal/ventral axis of the Drosophila embryo. However, the current techniques used to view …

In vivo imaging of intact Drosophila larvae at sub-cellular resolution

Y Zhang, P Füger, SB Hannan, JV Kern… - JoVE (Journal of …, 2010 - jove.com
Recent improvements in optical imaging, genetically encoded fluorophores and genetic
tools allowing efficient establishment of desired transgenic animal lines have enabled …

In-vivo centrifugation of Drosophila embryos

SL Tran, MA Welte - JoVE (Journal of Visualized Experiments), 2010 - jove.com
A major strategy for purifying and isolating different types of intracellular organelles is to
separate them from each other based on differences in buoyant density. However, when …