A simple PCR set-up for the detection of cytomegalovirus in clinical specimen was
developed. All components of the PCR master mix including Taq DNA polymerase, uracil N …

An in situ DNA hybridization kit for cytomegalovirus (CMV) was evaluated for the detection of
CMV in centrifugation culture. Of 61 clinical specimens, 17 (27.8%) were positive for CMV by …

Previous studies have shown that detection of cytomegalovirus (CMV) DNA in plasma is less
sensitive than the antigenemia assay for CMV surveillance in blood. In 1,983 blood samples …

Human cytomegalovirus (CMV) is a herpesvirus that is responsible for significant morbidity
and mortality in congenitally infected infants and immunocompromised patients. Antiviral …

We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus
(HCMV). The assay includes primer and probe sequences derived from conserved HCMV …

In this study, we compared the performance of six real-time PCR assays for the qualitative
detection of cytomegalovirus (CMV) in clinical samples other than plasma. Two hundred …

The polymerase chain reaction (PCR) technique offers a promising alternative to tissue
culture for the rapid and sensitive detection of cytomegalovirus (CMV) infection. However …

Several different primer-probe chemistries have been produced commercially for real-time
PCR detection and quantification of cytomegalovirus, but there are few studies evaluating …

ABSTRACT A duplex quantitative real-time PCR (qPCR) assay was designed to detect both
the polymerase gene (pol) and the glycoprotein gene (gB) of cytomegalovirus (CMV). The …

Direct detection of human cytomegalovirus (HCMV) from clinical specimens was examined
by using the polymerase chain reaction (PCR) for amplifying HCMV DNA. The efficiency of …