Stochasticity of TOL plasmid catabolic promoters sets a bimodal expression regime in Pseudomonas putida mt‐2 exposed to m‐xylene

R Silva‐Rocha, V de Lorenzo - Molecular microbiology, 2012 - Wiley Online Library
The expression dynamics of the catabolic promoters of the TOL plasmid pWW0 has been
examined in single cells of Pseudomonas putida mt‐2 exposed to m‐xylene. To this end, we …

Inferring the genetic network of m‐xylene metabolism through expression profiling of the xyl genes of Pseudomonas putida mt‐2

F Velázquez, V Parro, V de Lorenzo - Molecular microbiology, 2005 - Wiley Online Library
A subgenomic array of structural and regulatory genes of the TOL plasmid pWW0 of
Pseudomonas putida mt‐2 has been constructed to sort out the interplay between m‐xylene …

Early and late responses of TOL promoters to pathway inducers: identification of postexponential promoters in Pseudomonas putida with lacZ-tet bicistronic reporters

V De Lorenzo, I Cases, M Herrero… - Journal of …, 1993 - Am Soc Microbiol
Transcriptional lacZ fusions to the Pu and Pm promoters of the TOL (toluene degradation)
plasmid inserted in monocopy in the chromosome of Pseudomonas putida showed a very …

The regulatory logic of m‐xylene biodegradation by Pseudomonas putida mt‐2 exposed by dynamic modelling of the principal node Ps/Pr of the TOL plasmid

M Koutinas, MC Lam, A Kiparissides… - Environmental …, 2010 - Wiley Online Library
The structure of the extant transcriptional control network of the TOL plasmid pWW0 born by
Pseudomonas putida mt‐2 for biodegradation of m‐xylene is far more complex than one …

Decoding the genetic networks of environmental bacteria: regulatory moonlighting of the TOL system of Pseudomonas putida mt-2

R Silva-Rocha, D Pérez-Pantoja… - The ISME journal, 2013 - academic.oup.com
The first mechanistic description of gene expression control was proposed by Jacob and
Monod (1961) for the lac operon of E. coli. The first version of the model contemplated …

The logic layout of the TOL network of Pseudomonas putida pWW0 plasmid stems from a metabolic amplifier motif (MAM) that optimizes biodegradation of m-xylene

R Silva-Rocha, H de Jong, J Tamames… - BMC Systems Biology, 2011 - Springer
Background The genetic network of the TOL plasmid pWW0 of the soil bacterium
Pseudomonas putida mt-2 for catabolism of m-xylene is an archetypal model for …

Transcriptional tradeoff between metabolic and stress-response programs in Pseudomonas putida KT2440 cells exposed to toluene

P Domínguez-Cuevas, JE González-Pastor… - Journal of Biological …, 2006 - ASBMB
When Pseudomonas putida KT2440 cells encounter toluene in the growth medium, they
perceive it simultaneously as a potential nutrient to be metabolized, as a membrane …

Transcriptional induction kinetics from the promoters of the catabolic pathways of TOL plasmid pWW0 of Pseudomonas putida for metabolism of aromatics

S Marqués, A Holtel, KN Timmis… - Journal of bacteriology, 1994 - Am Soc Microbiol
We determined, under several growth conditions, the kinetics of mRNA synthesis from the
four Pseudomonas putida pWW0 plasmid promoters involved in degradation of xylenes and …

The rpoS gene regulates OP2, an operon for the lower pathway of xylene catabolism on the TOL plasmid, and the stress response in Pseudomonas putida mt-2

K Miura, S Inouye, A Nakazawa - Molecular and General Genetics MGG, 1998 - Springer
Operon OP2 on the Pseudomonas putida TOL plasmid encodes enzymes for m-toluate
catabolism; transcription of this operon is activated by XylS in the presence of m-toluate …

Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53

MT Gallegos, PA Williams, JL Ramos - Journal of bacteriology, 1997 - Am Soc Microbiol
The TOL plasmid pWW53 encodes a catabolic pathway for the metabolism of toluene. It
bears an upper-pathway operon for the oxidation of toluene to benzoate and a copy of the …