Base editing induces single-nucleotide changes in the DNA of living cells using a fusion
protein containing a catalytically defective Streptococcus pyogenes Cas9, a cytidine …

Base editing requires that the target sequence satisfy the protospacer adjacent motif
requirement of the Cas9 domain and that the target nucleotide be located within the editing …

Cytosine base editors (CBEs) enable targeted C• G-to-T• A conversions in genomic DNA.
Recent studies report that BE3, the original CBE, induces a low frequency of genome-wide …

Base editors (BEs) are RNA-guided CRISPR-Cas-derived genome editing tools that induce
single-nucleotide changes. The limitations of current BEs lie in their low precision …

We recently developed base editing, the programmable conversion of target C: G base pairs
to T: A without inducing double-stranded DNA breaks (DSBs) or requiring homology …

We describe base editors that combine both cytosine and adenine base-editing functions. A
codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase …

Applications of adenine base editors (ABEs) have been constrained by the limited
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …

The ability to precisely edit individual bases of bacterial genomes would accelerate the
investigation of the function of genes. Here we utilized a nickase Cas9-cytidine deaminase …

Although base editors are useful tools for precise genome editing, current base editors can
only convert either adenines or cytosines. We developed a dual adenine and cytosine base …

Base editing is a genome editing strategy that induces specific single-nucleotide changes
within genomic DNA. Two major DNA base editors, cytosine base editors and adenine base …