Isolation and suborganellar fractionation of Arabidopsis chloroplasts
Ú Flores-Pérez, P Jarvis - Isolation of Plant Organelles and Structures …, 2017 - Springer
Chloroplasts are structurally complex organelles containing~ 2000–3000 proteins. They are
delimited by a double membrane system or envelope, have an inner aqueous compartment …
delimited by a double membrane system or envelope, have an inner aqueous compartment …
Determining the location of an Arabidopsis chloroplast protein using in vitro import followed by fractionation and alkaline extraction
CC Chu, H Li - Chloroplast Research in Arabidopsis: Methods and …, 2011 - Springer
Chloroplasts have one of the most complicated structures among organelles. They have
three membrane systems, the outer and inner envelope membranes and the thylakoid …
three membrane systems, the outer and inner envelope membranes and the thylakoid …
Preparation of chloroplast sub-compartments from Arabidopsis for the analysis of protein localization by immunoblotting or proteomics
I Bouchnak, L Moyet, D Salvi, M Kuntz… - JoVE (Journal of …, 2018 - jove.com
Chloroplasts are major components of plant cells. Such plastids fulfill many crucial functions,
such as assimilation of carbon, sulfur and nitrogen as well as synthesis of essential …
such as assimilation of carbon, sulfur and nitrogen as well as synthesis of essential …
Isolation and preparation of chloroplasts from Arabidopsis thaliana plants
SE Kubis, KS Lilley, P Jarvis - 2D PAGE: Sample Preparation and …, 2008 - Springer
A major area of research in the postgenomic era has been the proteomic analysis of various
subcellular and suborganellar compartments. The success of these studies is to a large …
subcellular and suborganellar compartments. The success of these studies is to a large …
Purification and proteomic analysis of chloroplasts and their sub-organellar compartments
Sub-cellular proteomics has proven to be a powerful approach to link the information
contained in sequenced genomes from eukaryotic cells to the functional knowledge …
contained in sequenced genomes from eukaryotic cells to the functional knowledge …
Preparation of membrane fractions (envelope, thylakoids, grana, and stroma lamellae) from Arabidopsis chloroplasts for quantitative proteomic investigations and …
L Moyet, D Salvi, M Tomizioli… - … : Methods and Protocols, 2018 - Springer
Chloroplasts are semiautonomous organelles found in plants and protists. They are
surrounded by a double membrane system, or envelope. These envelope membranes …
surrounded by a double membrane system, or envelope. These envelope membranes …
Preparation of envelope membrane fractions from Arabidopsis chloroplasts for proteomic analysis and other studies
D Salvi, L Moyet, D Seigneurin-Berny, M Ferro… - Chloroplast Research in …, 2011 - Springer
Plastids are semiautonomous organelles restricted to plants and protists. These plastids are
surrounded by a double membrane system, or envelope. These envelope membranes …
surrounded by a double membrane system, or envelope. These envelope membranes …
Methodology: an optimized, high-yield tomato leaf chloroplast isolation and stroma extraction protocol for proteomics analyses and identification of chloroplast co …
O Bhattacharya, I Ortiz, LL Walling - Plant Methods, 2020 - Springer
Background Chloroplasts are critical organelles that perceive and convey metabolic and
stress signals to different cellular components, while remaining the seat of photosynthesis …
stress signals to different cellular components, while remaining the seat of photosynthesis …
Isolation of chloroplasts from plant protoplasts
Chloroplasts can be isolated from higher plants directly following homogenization; however,
the resulting yield, purity, and intactness are often low, necessitating a large amount of …
the resulting yield, purity, and intactness are often low, necessitating a large amount of …
AT_CHLORO: the first step when looking for information about subplastidial localization of proteins
D Salvi, S Bournais, L Moyet, I Bouchnak… - Plastids: Methods and …, 2018 - Springer
Plastids contain several key subcompartments. The two limiting envelope membranes (inner
and outer membrane of the plastid envelope with an intermembrane space between), an …
and outer membrane of the plastid envelope with an intermembrane space between), an …