A gene encoding the pollen major allergen Bet v 1 fromBetula verrucosa (White Birch) has
been cloned and expressed inEscherichia colias a fusion with maltose-binding protein and …

Bet v 1 is the major birch pollen allergen. A highly efficient expression and purification
scheme for mutant forms of this protein was developed on the basis of the pET expression …

Bet v 1, the major allergen of birch pollen, displays a considerable degree of heterogeneity.
Several charge variants have been detected by two-dimensional IgE immunoblots and …

Birch pollen allergy is predominantly caused by the major allergen Bet v 1 and can lead to
crossreactions with homologous proteins in food. Two major cross‐reactive food allergens …

Pollen from trees of the order Fagales (eg birch, alder, hazel, oak, and hornbeam) are a
major cause of Type I allergies observed in early spring. Previously, we reported the cloning …

Bet v 1, the single major allergen from birch pollen, shares IgE epitopes with all major tree
pollen allergens from closely related species such as alder, hazel, hornbeam, beech, and …

In 2D-PAGE analysis of Bet v 1, the major birch pollen allergen, up to 12 isoforms can be
demonstrated that differ in their isoelectric points from about pH 4.9 to pH 5.9. The molecular …

Three isoforms of the major birch pollen allergen, Bet v, 1 from Betula verrucosa have been
expressed as recombinant proteins in E. coli and purified. The immunochemical properties …

Background: We describe the production in Escherichia coli as a recombinant protein of
clinical grade wild-type Bet v 1a (rBet v 1a), to be used as a candidate vaccine against birch …

The gene encoding the major allergen, Bet v I, from Betula verrucosa (white birch) pollen
was cloned by application of the polymerase chain reaction with double-stranded cDNA as …