Directed evolution of TetR for constructing sensitive and broad-spectrum tetracycline antibiotics whole-cell biosensor

S Li, D Chen, Z Liu, S Tao, T Zhang, Y Chen… - Journal of Hazardous …, 2023 - Elsevier
Antibiotic abuse is the main reason for the drug resistance of pathogenic bacteria, posing a
potential health risk. Antibiotic surveillance is critical for preventing antibiotic contamination …

Construction of an extended range whole-cell tetracycline biosensor by use of the tet(M) resistance gene

MI Bahl, LH Hansen, SJ Sørensen - FEMS Microbiology Letters, 2005 - academic.oup.com
An extended range whole-cell tetracycline biosensor strain was constructed by insertion of
the tet (M) gene, encoding tetracycline resistance by ribosomal protection, into plasmid …

Development of transcriptional factor-based whole-cell biosensors to monitor and degrade antibiotics using mutant cells obtained via adaptive laboratory evolution

J Li, Z Qin, B Zhang, X Wu, J Wu, L Peng… - Journal of Hazardous …, 2024 - Elsevier
With the widespread use of antibiotics and increasing environmental concerns regarding
antibiotic abuse, the detection and degradation of antibiotic residues in various samples has …

A ratiometric dual-fluorescent paper-based synthetic biosensor for visual detection of tetracycline on-site

Y Liu, Y Wu, L Wang, L Zhu, Y Dong, W Xu - Journal of Hazardous Materials, 2024 - Elsevier
The excessive use of tetracycline poses a threat to human health, making it essential to
monitor and regulate its usage. While whole-cell biosensors offer a simple and cost-effective …

Comparison of three genetically modified Escherichia coli biosensor strains for amperometric tetracycline measurement

W Song, N Pasco, R Gooneratne, RJ Weld - Biosensors and Bioelectronics, 2012 - Elsevier
Three separate genetic strategies, based upon the induced expression of three different
genes (lacZ, selA and nuoA) were tested to provide the SciTox assay with sensitive and …

[HTML][HTML] A genetically engineered Escherichia coli that senses and degrades tetracycline antibiotic residue

Z Mu, Z Zou, Y Yang, W Wang, Y Xu, J Huang… - Synthetic and Systems …, 2018 - Elsevier
Due to the abuse of antibiotics, antibiotic residues can be detected in both natural
environment and various industrial products, posing threat to the environment and human …

Monitoring tetracycline through a solid-state nanopore sensor

Y Zhang, Y Chen, Y Fu, C Ying, Y Feng, Q Huang… - Scientific Reports, 2016 - nature.com
Antibiotics as emerging environmental contaminants, are widely used in both human and
veterinary medicines. A solid-state nanopore sensing method is reported in this article to …

Entwicklung eines optischen Biosensor-Assays mit Elementen aus der Resistenzforschung zum Screening auf Tetracyclin-Rückstände in Lebensmitteln

N Möller - 2007 - elpub.bib.uni-wuppertal.de
A tetracycline specific-screening assay for residue analysis in honey and raw milk using the
surface plasmon resonance (SPR) technology is described. For this purpose the principle of …

Sensitive and fast detection of tetracycline using an aptasensor

SH Jalalian, SM Taghdisi, NM Danesh, H Bakhtiari… - Analytical …, 2015 - pubs.rsc.org
Tetracycline (TET) is one of the most extensively used antibiotics in humans and animals.
Fast and sensitive measurement of TET is of great interest. In this study, a triple-helix …

Development of an epigenetic tetracycline sensor system based on DNA methylation

T Ullrich, S Weirich, A Jeltsch - PLoS One, 2020 - journals.plos.org
Bacterial live cell sensors are potentially powerful tools for the detection of environmental
toxins. In this work, we have established and validated a flow cytometry readout for an …