Protein trans-splicing using split inteins is a powerful and convenient reaction to chemically
modify recombinantly expressed proteins under mild conditions. In particular, semisynthetic …

Protein trans‐splicing catalyzed by split inteins is a powerful technique for assembling a
polypeptide backbone from two separate parts. However, split inteins with robust efficiencies …

Conventional split inteins have been useful for trans-splicing between recombinant proteins,
and an artificial S1 split intein is useful for adding synthetic peptide onto the N terminus of …

We recently reported an artificially split intein based on the Ssp DnaB mini-intein that
consists of a synthetic N-terminal intein fragment (Int N) and a recombinant C-terminal part …

Protein trans-splicing by split inteins holds great potential for the chemical modification and
semisynthesis of proteins. However, the structural requirements of the extein sequences …

This volume focuses on applications of split inteins, and the progress that has been made in
the past 5 years on discovery and engineering of fast and more efficient split inteins. The first …

Protein trans-splicing by split inteins has many uses in protein production and research.
Splicing proteins with synthetic peptides, which employs atypical split inteins, is particularly …

Chemoselective and regioselective chemical protein labeling is of great importance, yet no
current technique is sufficiently general and simple to perform. Protein trans‐splicing by split …

Chemical-tag labeling of proteins involving split inteins is an approach for the selective
chemical modification of proteins without the requirement of any chemical synthesis to be …

Protein splicing is an autocatalytic reaction in which an internal protein domain, the intein,
excises itself out of a precursor protein and concomitantly links the two flanking sequences …