Molecular scissors engineered for site-specific modification of the genome hold great
promise for effective functional analyses of genes, genomes and epigenomes and could …

Clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 9
(Cas9) is one of the most versatile and efficient gene editing technologies, which is derived …

The ability to change the genetic information of immune cells is a powerful tool for basic and
clinical settings. CRISPR/Cas9 gene editing technology by providing an efficient approach …

Gene therapy represents a significant potential to revolutionize the field of hematology with
applications in correcting genetic mutations, generating cell lines and animal models, and …

Genomic editing to correct disease-causing mutations is a promising approach for the
treatment of human diseases. As a simple and programmable nuclease-based genomic …

Precise nucleic acid editing technologies have facilitated the research of cellular function
and the development of novel therapeutics, especially the current programmable nucleases …

Highlights•Unwanted on-target mutation occurs after CRISPR–Cas9 cleavage.•Assessment
of comprehensive on-target outcomes is necessary.•Clinical genomic engineering requires …

This is a spectacular moment for genetics to evolve in genome editing, which encompasses
the precise alteration of the cellular DNA sequences within various species. One of the most …

The clustered regularly interspaced short palindromic repeats system has demonstrated
considerable advantages over other nuclease-based genome editing tools due to its high …

Chronic myeloid leukemia (CML) is a hematopoietic malignancy produced by a unique
oncogenic event involving the constitutively active tyrosine-kinase (TK) BCR/ABL1. TK …