[HTML][HTML] A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system
CRISPR/Cas9 can be used as an experimental tool to inactivate genes in cells. However, a
CRISPR-targeted cell population will not show a uniform genotype of the targeted gene …
CRISPR-targeted cell population will not show a uniform genotype of the targeted gene …
[HTML][HTML] Efficient CRISPR/Cas9-mediated gene knockin in mouse hematopoietic stem and progenitor cells
Mutations accumulating in hematopoietic stem and progenitor cells (HSPCs) during
development can cause severe hematological disorders. Modeling these mutations in mice …
development can cause severe hematological disorders. Modeling these mutations in mice …
Genome Engineering of Hematopoietic Stem Cells Using CRISPR/Cas9 System
N Devaraju, V Rajendiran, NS Ravi… - Stem Cell Assays …, 2022 - Springer
Ex vivo genetic manipulation of autologous hematopoietic stem and progenitor cells
(HSPCs) is a viable strategy for the treatment of hematologic and primary immune disorders …
(HSPCs) is a viable strategy for the treatment of hematologic and primary immune disorders …
[HTML][HTML] Integration of CRISPR/Cas9 with artificial intelligence for improved cancer therapeutics
Gene editing has great potential in treating diseases caused by well-characterized
molecular alterations. The introduction of clustered regularly interspaced short palindromic …
molecular alterations. The introduction of clustered regularly interspaced short palindromic …
[HTML][HTML] From Descriptive to Functional Genomics of Leukemias Focusing on Genome Engineering Techniques
Genome engineering makes the precise manipulation of DNA sequences possible in a cell.
Therefore, it is essential for understanding gene function. Meganucleases were the start of …
Therefore, it is essential for understanding gene function. Meganucleases were the start of …
[HTML][HTML] Whole-genome sequencing analysis reveals high specificity of CRISPR/Cas9 and TALEN-based genome editing in human iPSCs
C Smith, A Gore, W Yan, L Abalde-Atristain, Z Li, C He… - Cell stem cell, 2014 - cell.com
Human iPSCs provide renewable cell sources for human biology and disease research and
the potential for developing gene and cell therapy. Realization of this potential will rely in …
the potential for developing gene and cell therapy. Realization of this potential will rely in …
[HTML][HTML] Key challenges in bringing CRISPR-mediated somatic cell therapy into the clinic
Editorial summary Genome editing using clustered regularly interspersed short palindromic
repeats (CRISPR) and CRISPR-associated proteins offers the potential to facilitate safe and …
repeats (CRISPR) and CRISPR-associated proteins offers the potential to facilitate safe and …
[HTML][HTML] CRISPR-Cas9 genome editing induces megabase-scale chromosomal truncations
G Cullot, J Boutin, J Toutain, F Prat… - Nature …, 2019 - nature.com
CRISPR-Cas9 is a promising technology for genome editing. Here we use Cas9 nuclease-
induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital …
induced double-strand break DNA (DSB) at the UROS locus to model and correct congenital …
[HTML][HTML] Programmable molecular scissors: applications of a new tool for genome editing in biotech
Targeted genome editing is an advanced technique that enables precise modification of the
nucleic acid sequences in a genome. Genome editing is typically performed using tools …
nucleic acid sequences in a genome. Genome editing is typically performed using tools …
[HTML][HTML] COSMID: a web-based tool for identifying and validating CRISPR/Cas off-target sites
Precise genome editing using engineered nucleases can significantly facilitate biological
studies and disease treatment. In particular, clustered regularly interspaced short …
studies and disease treatment. In particular, clustered regularly interspaced short …