Metabolomics: Methods and Protocols examines the state-of-the-art in metabolomic
analysis. Leading researchers in the field present protocols for the application of …

Highlights•We review high-throughput mass spectrometry-based methods for metabolomic
analysis.•High-throughput approaches usually improve throughput at the expense of …

Direct injection of samples on high-resolving mass spectrometers is an effective way to
maximize analytical throughput and yet allow analyte discrimination in complex samples by …

The chemical identification of mass spectrometric signals in metabolomic applications is
important to provide conversion of analytical data to biological knowledge about metabolic …

Highlights•Recent LC–MS and GC–MS methods are reviewed.•The use of multiple columns
extends the coverage of the metabolome.•Super critical fluid chromatography (SFC) could …

A method for the preparation and GC− TOF-MS analysis of human serum samples has been
developed and evaluated for application in long-term metabolomic studies. Serum samples …

Metabolomics is the comprehensive profiling of the small molecule composition of a
biological sample. Since metabolites are often the indirect products of gene expression, this …

Small molecules, including metabolites and lipids, provide information on metabolic
pathways and active biological processes in living organisms. They are often diagnostic of …

Metabolomics studies rely on the analysis of the multitude of small molecules (metabolites)
present in a biological system. Most commonly, metabolomics is heavily supported by mass …

Metabolomics aims at detection and quantitation of all metabolites in biological samples.
The presence of metabolites with a wide variety of physicochemical properties and different …