Routine methods for the evaluation of sperm quality are not sufficiently useful to determine
the sensitivity of sperm cells to cold shock. The aim of our preliminary study was to …

Throughout cryopreservation, sperm are exposed to major osmotic challenges. Only intact
membranes of sperm cells are able to regulate these volumetric changes, which can be …

This work was carried out to test whether viability of pig spermatozoa subjected to an
osmotic test is correlated to sperm cryosurvival. Spermatozoa were cooled from 22° C to-5° …

The objective of this study was to assess the sperm membrane integrity and permeability of
frozen-thawed bovine spermatozoa, processed at varying temperatures during and after …

Semen cryopreservation results in sublethal damage to sperm due to membrane
deterioration and an increased number of spermatozoa that undergo acrosome reaction …

In this study, frozen semen samples from 3 bulls were thawed at 37, 40 and 70° C for 20, 15
and 5 seconds, respectively. Sperm membrane integrity and motility were analysed using …

Boar semen is occasionally transferred to different locations in liquid form at 15° C for
cryopreservation. However, the use of frozen boar semen is limited due to the high …

The objective of this study was to evaluate the effects of thawing and processing
temperatures on post-thaw sperm viability, occurrence of osmotic shock and sperm …

Cryopreservation imposes irreversible damage to sperm membranes, such as swelling and
disruption of plasma and acrosome membranes, changes in membrane fluidity, altered …

A recently developed triple staining (SNARF-1/YO-PRO-1/ethidium homodimer) was used to
assess early changes in boar sperm membrane integrity (MI) with the results of …