Full-length RNA-seq from single cells using Smart-seq2
Emerging methods for the accurate quantification of gene expression in individual cells hold
promise for revealing the extent, function and origins of cell-to-cell variability. Different high …
promise for revealing the extent, function and origins of cell-to-cell variability. Different high …
Smart-seq2 for sensitive full-length transcriptome profiling in single cells
Single-cell gene expression analyses hold promise for characterizing cellular heterogeneity,
but current methods compromise on either the coverage, the sensitivity or the throughput …
but current methods compromise on either the coverage, the sensitivity or the throughput …
Single-cell RNA counting at allele and isoform resolution using Smart-seq3
Large-scale sequencing of RNA from individual cells can reveal patterns of gene, isoform
and allelic expression across cell types and states. However, current short-read single-cell …
and allelic expression across cell types and states. However, current short-read single-cell …
Full-length single-cell RNA sequencing with smart-seq2
S Picelli - Single Cell Methods: Sequencing and Proteomics, 2019 - Springer
In the last few years single-cell RNA sequencing (scRNA-seq) has enabled the investigation
of cellular heterogeneity at the transcriptional level, the characterization of rare cell types as …
of cellular heterogeneity at the transcriptional level, the characterization of rare cell types as …
Exponential scaling of single-cell RNA-seq in the past decade
Measurement of the transcriptomes of single cells has been feasible for only a few years, but
it has become an extremely popular assay. While many types of analysis can be carried out …
it has become an extremely popular assay. While many types of analysis can be carried out …
Entering the era of single-cell transcriptomics in biology and medicine
R Sandberg - Nature methods, 2014 - nature.com
Recent technical advances have enabled RNA sequencing (RNA-seq) in single cells.
Exploratory studies have already led to insights into the dynamics of differentiation, cellular …
Exploratory studies have already led to insights into the dynamics of differentiation, cellular …
Quantitative single-cell RNA-seq with unique molecular identifiers
Single-cell RNA sequencing (RNA-seq) is a powerful tool to reveal cellular heterogeneity,
discover new cell types and characterize tumor microevolution. However, losses in cDNA …
discover new cell types and characterize tumor microevolution. However, losses in cDNA …
Effective detection of variation in single-cell transcriptomes using MATQ-seq
The quantification of transcriptional variation in single cells, particularly within the same cell
population, is currently limited by the low sensitivity and high technical noise of single-cell …
population, is currently limited by the low sensitivity and high technical noise of single-cell …
Introduction to single‐cell RNA sequencing
TK Olsen, N Baryawno - Current protocols in molecular biology, 2018 - Wiley Online Library
During the last decade, high‐throughput sequencing methods have revolutionized the entire
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …
field of biology. The opportunity to study entire transcriptomes in great detail using RNA …
Single-cell quantification of a broad RNA spectrum reveals unique noncoding patterns associated with cell types and states
The ability to interrogate total RNA content of single cells would enable better mapping of
the transcriptional logic behind emerging cell types and states. However, current single-cell …
the transcriptional logic behind emerging cell types and states. However, current single-cell …