Many types of mammalian cells can aggregate and differentiate into 3‐D multicellular
spheroids when cultured in suspension or a nonadhesive environment. Compared to …

Introduction: Developing in vitro models for studying cell biology and cell physiology is of
great importance to the fields of biotechnology, cancer research, drug discovery, toxicity …

Spheroids are increasingly being employed to answer a wide range of clinical and
biomedical inquiries ranging from pharmacology to disease pathophysiology, with the …

Three-dimensional multicellular spheroids (MCSs) have received extensive attention in the
field of biomedicine due to their ability to simulate the structure and function of tissues in vivo …

Spheroids provide a 3D environment with intensive cell–cell contacts. As a result of their
excellent regenerative properties and rapid progress in their high-throughput production …

Abstract Three-dimensional (3D) cell culture has tremendous advantages to closely mimic
the in vivo architecture and microenvironment of healthy tissue and organs, as well as of …

Multi‐cellular spheroids are formed as a 3D structure with dense cell–cell/cell–extracellular
matrix interactions, and thus, have been widely utilized as implantable therapeutics and …

Three-dimensional multicellular spheroids (MCSs) have a complex architectural structure,
dynamic cell–cell/cell–matrix interactions and bio-mimicking in vivo microenvironment. As a …

Abstract 3D cell culture has been strongly advocated as a highly useful culture technique
instead of the traditional 2D cell culture. Specially, spheroids, as the primary mode of 3D cell …

Cellular spheroids are aggregates of cells that are being explored to address fundamental
biological questions and as building blocks for engineered tissues. Spheroids possess …