Systematic analysis of the factors that adversely affect the rate of cell accumulation in mouse embryos during their culture in vitro

XL Jin, C O'Neill - Reproductive Biology and Endocrinology, 2014 - Springer
Background Retarded embryo growth is a pervasive effect of culture in vitro. Methods A
systematic analysis of the interactions between media design, embryo culture density …

Exogenous growth factors do not affect the development of individually cultured murine embryos

JR Herrick, AF Greene-Ermisch, WB Schoolcraft… - Journal of Assisted …, 2018 - Springer
Purpose The objective of this study was to evaluate the effects of multiple growth factors on
the development of individually cultured murine embryos. Methods Embryos produced by in …

The effect of culture medium volume on in vitro development of mouse embryos

MAA KARIMPOUR, MA ESMAEILNEZHAD - 2005 - sid.ir
Background: In the field of mammalian embryo culture, the putative influence of
autocrine/paracrine factor (s), produce by the embryos itself, is under investigation. A smaller …

[PDF][PDF] Analysis of gene transcription alterations at the blastocyst stage related to the long-term consequences of in vitro culture in mice

R Fernandez-Gonzalez, J de Dios Hourcade… - …, 2009 - researchgate.net
We have reported that in vitro culture (IVC) of preimplantation mouse embryos in the
presence of FCS produces long-term effects (LTE) on development, growth and behaviour of …

Recent advances in the production of viable human embryos in vitro

TB Pool - Reproductive BioMedicine Online, 2002 - Elsevier
The introduction of stress to embryonic blastomeres through inappropriate culture conditions
results ultimately in the loss of viability. Retention of normal metabolic function in human …

Effect of incubation volume and embryo density on the development and viability of mouse embryos in vitro

M Lane, DK Gardner - Human reproduction, 1992 - academic.oup.com
The morphology, cleavage rate and viability of preimplantation embryos from random bred
Swiss mice were assessed after culture in different incubation volumes and embryo …

A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos

AF Ermisch, JR Herrick, R Pasquariello, MKC Dyer… - Scientific reports, 2020 - nature.com
Further refinement of culture media is needed to improve the quality of embryos generated
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …

[HTML][HTML] Media optimization to promote rat embryonic development to the blastocyst stage in vitro

H Men, BJ Stone, EC Bryda - Theriogenology, 2020 - Elsevier
Efficient model production in rats that incorporates newly developed genetic editing and
embryo transfer tools, such as CRISPR/Cas9 technology and non-surgical embryo transfer …

In vitro development of one-cell embryos from outbred mice: influence of culture medium composition

A Spindle - In vitro cellular & developmental biology, 1990 - Springer
One-cell embryos from outbred mice (CF1, CD-1, and Dub: ICR) were cultured in various
modifications of egg culture medium (ECM). The best development was observed in medium …

Culture of preimplantation embryos and its long-term effects on gene expression and phenotype

S Khosla, W Dean, W Reik, R Feil - Human reproduction update, 2001 - academic.oup.com
A growing number of medical, scientific and biotechnological procedures rely on culture of
mammalian preimplantation embryos. This review presents currently available data on …