Background Whole embryo culture is a valuable research method in mammalian
developmental biology and birth defects research, enabling longitudinal studies of …

Since the inception of modern embryo culture media over 50 years ago there have been
significant developments in culture systems for the mammalian preimplantation embryo …

One‐cell embryos from some inbred and random‐bred mice, but not those derived from
certain F1 hybrids, suffer from a block during in vitro development known as the two‐cell …

During infertility treatment with IVF, embryos are cultured either in groups or individually.
Each approach has potential benefits and detriments, and the purpose of this review is to try …

The role of amino acids, ethylenediaminetetraacetic acid (EDTA), transferrin, oxygen,
glucose, glutamine, taurine and ammonium in CF1 mouse zygote development in culture …

A limiting factor in the development of new technologies and transport of rats worldwide has
been the inability to robustly culture preimplantation embryos. Previously, culture in vitro to …

Embryos are routinely cultured individually, although this can reduce blastocyst
development. Culture in atmospheric (20%) oxygen is also common, despite multiple …

Although the mouse is widely used as an animal model for the in vitro culture of human
preimplantation embryos, arguments can be made for the validity of many domestic species …

Objective Validate single versus sequential culture media for murine embryo development.
Design Prospective laboratory experiment. Setting Assisted Reproduction Laboratory …

We have recently reported the use of sequential simplex optimization methods to design a
medium (SOM) that overcomes the block to development beyond two cells which occurs in …