State-of-the-art methods in high-resolution three-dimensional optical microscopy require that
the focus be scanned through the entire region of interest. However, an analysis of the …

High-resolution real-time tomography of scattering tissues is important for many areas of
medicine and biology,,,,,. However, the compromise between transverse resolution and …

Light microscopy of thick biological samples, such as tissues, is often limited by aberrations
caused by refractive index variations within the sample itself. This problem is particularly …

We describe the construction and use of a compact dual-view inverted selective plane
illumination microscope (diSPIM) for time-lapse volumetric (4D) imaging of living samples at …

Improving the spatial resolution of optical microscopes is important for a vast number of
applications in the life sciences. Optical microscopy allows intact samples and living cells to …

We present spatial light interference tomography (SLIT), a label-free method for 3D imaging
of transparent structures such as live cells. SLIT uses the principle of interferometric imaging …

We introduce and demonstrate a new microscopy concept: imaging interferometric
microscopy (IIM), which is related to holography, synthetic-aperture imaging, and off-axis …

The axial resolution of three-dimensional structured illumination microscopy (3D SIM) is
limited to∼ 300 nm. Here we present two distinct, complementary methods to improve axial …

The resolution of an optical microscope is fundamentally limited by diffraction. In a
conventional wide-field fluorescence microscope, the resolution limit is at best 200 nm …

We report that single (or selective) plane illumination microscopy (SPIM), combined with a
new deconvolution algorithm, provides a three-dimensional spatial resolution exceeding …