Type IV pilus (T4P) systems are complex molecular machines that polymerize major pilin
proteins into thin filaments displayed on bacterial surfaces. Pilus functions require rapid …

The type 2 secretion system (T2SS), a multi-protein machinery that spans both the inner and
the outer membranes of Gram-negative bacteria, is used for the secretion of several critically …

Many gram-negative bacteria produce thin protein filaments, named pili, which extend
beyond the confines of the outer membrane. The importance of these pili is illustrated by the …

The Pseudomonas aeruginosa inner membrane protein FimV is among several proteins of
unknown function required for type IV pilus-mediated twitching motility, arising from …

Bacterial virulence factors are typically surface-associated or secreted molecules that in
Gram-negative bacteria must cross the outer membrane (OM). Protein translocation across …

The Klebsiella oxytoca pullulanase secreton (type II secretion system) components PulM
and PulL were tagged at their N termini with green fluorescent protein (GFP), and their …

A cluster of 14 genes located on the large plasmid of enteropathogenic Escherichia coli
(EPEC) strains is sufficient to direct the biogenesis of the type IV bundle-forming pilus (BFP) …

Bacterial type IV pili are critical for diverse biological processes including horizontal gene
transfer, surface sensing, biofilm formation, adherence, motility, and virulence. These …

EpsE is a cytoplasmic component of the type II secretion system in Vibrio cholerae. Through
ATP hydrolysis and an interaction with the cytoplasmic membrane protein EpsL, EpsE …

Uropathogenic strains of Escherichia coli assemble type 1 and P pili to colonize the bladder
and kidney respectively. These pili are prototype structures assembled by the …