Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence mutation detection in circulating tumour DNA
V Rowlands, AJ Rutkowski, E Meuser, TH Carr… - Scientific Reports, 2019 - nature.com
Liquid biopsies offer the potential to monitor cancer response and resistance to therapeutics
in near real-time. However, the plasma cell free DNA (cfDNA) level can be low and the …
in near real-time. However, the plasma cell free DNA (cfDNA) level can be low and the …
Preanalytical blood sample workup for cell‐free DNA analysis using droplet digital PCR for future molecular cancer diagnostics
JH van Ginkel, DA van den Broek, J van Kuik… - Cancer …, 2017 - Wiley Online Library
In current molecular cancer diagnostics, using blood samples of cancer patients for the
detection of genetic alterations in plasma (cell‐free) circulating tumor DNA (ct DNA) is an …
detection of genetic alterations in plasma (cell‐free) circulating tumor DNA (ct DNA) is an …
Applying standard clinical chemistry assay validation to droplet digital PCR quantitative liquid biopsy testing
D Milosevic, JR Mills, MB Campion… - Clinical …, 2018 - academic.oup.com
Abstract BACKGROUND Droplet digital PCR (ddPCR) is an emerging technology for
quantitative cell-free DNA oncology applications. However, assay performance criteria must …
quantitative cell-free DNA oncology applications. However, assay performance criteria must …
Current and emerging applications of droplet digital PCR in oncology
S Olmedillas-López, M García-Arranz… - Molecular diagnosis & …, 2017 - Springer
The clinical management of cancer has evolved in recent years towards more personalized
strategies that require a comprehensive knowledge of the complex molecular features …
strategies that require a comprehensive knowledge of the complex molecular features …
[HTML][HTML] Detecting circulating tumor DNA in hepatocellular carcinoma patients using droplet digital PCR is feasible and reflects intratumoral heterogeneity
A Huang, X Zhang, SL Zhou, Y Cao, XW Huang… - Journal of …, 2016 - ncbi.nlm.nih.gov
Purpose: Circulating tumor DNA (ctDNA) is increasingly recognized as liquid biopsy to
profile tumor genome. Droplet digital PCR (ddPCR) is a highly sensitive and easily operable …
profile tumor genome. Droplet digital PCR (ddPCR) is a highly sensitive and easily operable …
Droplet-based digital PCR and next generation sequencing for monitoring circulating tumor DNA: a cancer diagnostic perspective
M Postel, A Roosen, P Laurent-Puig… - Expert review of …, 2018 - Taylor & Francis
Introduction: Early detection of cancers through the analysis of ctDNA could have a
significant impact on morbidity and mortality of cancer patients. However, using ctDNA for …
significant impact on morbidity and mortality of cancer patients. However, using ctDNA for …
Droplet-based digital PCR: application in cancer research
The efficient characterization of genetic and epigenetic alterations in oncology, virology, or
prenatal diagnostics requires highly sensitive and specific high-throughput approaches …
prenatal diagnostics requires highly sensitive and specific high-throughput approaches …
Current and emerging applications of droplet digital PCR in oncology: an updated review
S Olmedillas-López, R Olivera-Salazar… - Molecular Diagnosis & …, 2022 - Springer
In the era of personalized medicine and targeted therapies for the management of patients
with cancer, ultrasensitive detection methods for tumor genotyping, such as next-generation …
with cancer, ultrasensitive detection methods for tumor genotyping, such as next-generation …
The clinical utility of droplet digital PCR for profiling circulating tumor DNA in breast cancer patients
U Gezer, AJ Bronkhorst, S Holdenrieder - Diagnostics, 2022 - mdpi.com
Breast cancer is the most common cancer affecting women worldwide. It is a malignant and
heterogeneous disease with distinct molecular subtypes, which has prognostic and …
heterogeneous disease with distinct molecular subtypes, which has prognostic and …
Clinical utility of liquid biopsy-based actionable mutations detected via ddPCR
Cancer is one of the leading causes of death worldwide and remains a major public health
challenge. The introduction of more sensitive and powerful technologies has permitted the …
challenge. The introduction of more sensitive and powerful technologies has permitted the …