IHF protein inhibits cleavage but not assembly of plasmid R388 relaxosomes

G Moncalián, M Valle, JM Valpuesta… - Molecular …, 1999 - Wiley Online Library
Relaxosomes are specific nucleoprotein structures involved in DNA‐processing reactions
during bacterial conjugation. In this work, we present evidence indicating that plasmid R388 …

Localized denaturation of oriT DNA within relaxosomes of the broad‐host‐range plasmid R1162

S Zhang, RJ Meyer - Molecular microbiology, 1995 - Wiley Online Library
The broad‐host‐range, multicopy plasmid R1162 is efficiently mobilized during conjugation
by the self‐transmissible plasmid R751. The relaxosome, a complex of plasmid DNA and …

Two atypical mobilization proteins are involved in plasmid CloDF13 relaxation

B Núñez, F De La Cruz - Molecular microbiology, 2001 - Wiley Online Library
The mobilization region of plasmid CloDF13 was localized to a 3.6 kb DNA segment that
was analysed by transposon mutagenesis and DNA sequencing. Analysis of the DNA …

MobB protein stimulates nicking at the R1162 origin of transfer by increasing the proportion of complexed plasmid DNA

T Perwez, R Meyer - Journal of bacteriology, 1996 - Am Soc Microbiol
An essential early step in conjugal mobilization of R1162, nicking of the DNA strand that is
subsequently transferred, is carried out in the relaxosome, a complex of two plasmid …

TraY and Integration Host Factor oriT Binding Sites and F Conjugal Transfer: Sequence Variations, but Not Altered Spacing, Are Tolerated

SL Williams, JF Schildbach - Journal of bacteriology, 2007 - Am Soc Microbiol
Bacterial conjugation is the process by which a single strand of a conjugative plasmid is
transferred from donor to recipient. For F plasmid, TraI, a relaxase or nickase, binds a single …

A new domain of conjugative relaxase TrwC responsible for efficient oriT‐specific recombination on minimal target sequences

CE César, C Machón, F De La Cruz… - Molecular …, 2006 - Wiley Online Library
We show that relaxase TrwC promotes recombination between two directly repeated oriTs
while related relaxases TraI of F and pKM101 do not. Efficient recombination required also …

Relaxosome function and conjugation regulation in F‐like plasmids–a structural biology perspective

JJW Wong, J Lu, JNM Glover - Molecular microbiology, 2012 - Wiley Online Library
The tra operon of the prototypical F plasmid and its relatives enables transfer of a copy of the
plasmid to other bacterial cells via the process of conjugation. Tra proteins assemble to form …

[HTML][HTML] Stepwise assembly of a relaxosome at the F plasmid origin of transfer

MT Howard, WC Nelson, SW Matson - Journal of Biological Chemistry, 1995 - ASBMB
A central step in the transfer of genetic information during bacterial conjugation of the
Escherichia coli F plasmid involves the formation of a protein-DNA complex, called the …

Genes involved in conjugative DNA processing of plasmid R6K

B Núñez, P Avila, F De La Cruz - Molecular microbiology, 1997 - Wiley Online Library
The conjugative transfer region of the IncX plasmid R6K (TRAX) was analysed by
transposon mutagenesis and DNA sequencing. Tn5tac1 insertional mutations localized …

[HTML][HTML] Site-specific integration of foreign DNA into minimal bacterial and human target sequences mediated by a conjugative relaxase

L Agúndez, C González-Prieto, C Machón, M Llosa - PloS one, 2012 - journals.plos.org
Background Bacterial conjugation is a mechanism for horizontal DNA transfer between
bacteria which requires cell to cell contact, usually mediated by self-transmissible plasmids …