The Tra1 region of broad host range IncPα plasmid RK2 encodes proteins essential for its
promiscuous conjugative transfer and includes oriT, the site at which nicking occurs to …

Plasmids containing a direct repeat of plasmid R388 oriT are capable of site-specific
recombination, which results in deletion of the intervening DNA. This reaction occurs in the …

The DNA transfer stage of conjugation requires the products of the F sex factor genes
traMYDIZ and the cis-acting site oriT. Previous interpretation of genetic and protein analyses …

Assemblies of plasmid‐encoded proteins direct the conjugative transfer of plasmid DNA
molecules between bacteria. These include the membrane‐associated mating pair …

Transfer of conjugative plasmids requires relaxases, proteins that cleave one plasmid strand
sequence specifically. The F plasmid relaxase TraI (1,756 amino acids) is also a highly …

The effect of TraY protein on TraI-catalyzed strand scission at the R1 transfer origin (oriT) in
vivo was investigated. As expected, the cleavage reaction was not detected in Escherichia …

Protein TrwC is the conjugative relaxase responsible for DNA processing in plasmid R388
bacterial conjugation. TrwC has two catalytic tyrosines, Y18 and Y26, both able to carry out …

The origin of plasmid DNA transfer, oriT, has been localized on RK2, a conjugative drug-
resistance plasmid of the IncP group with a very broad host range in gram-negative bacteria …

Conjugative transfer of the self-transmissible IncP plasmid RP4 requires the product of the
RP4 traK gene. By using the phage T7 expression system, the traK gene product was …

TraI relaxase–helicase is the central catalytic component of the multiprotein relaxosome
complex responsible for conjugative DNA transfer (CDT) between bacterial cells. CDT is a …