Large-scale generation and analysis of filamentous fungal DNA barcodes boosts coverage for kingdom fungi and reveals thresholds for fungal species and higher …
Species identification lies at the heart of biodiversity studies that has in recent years
favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source …
favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source …
Utilizing ITS1 and ITS2 to study environmental fungal diversity using pyrosequencing
C Monard, S Gantner, J Stenlid - FEMS microbiology ecology, 2013 - academic.oup.com
The shorter reads generated by high-throughput sequencing has led to a focus on either the
ITS1 or the ITS2 sublocus in fungal diversity analyses. Our study aimed to determine how …
ITS1 or the ITS2 sublocus in fungal diversity analyses. Our study aimed to determine how …
Sequence depth, not PCR replication, improves ecological inference from next generation DNA sequencing
DP Smith, KG Peay - PloS one, 2014 - journals.plos.org
Recent advances in molecular approaches and DNA sequencing have greatly progressed
the field of ecology and allowed for the study of complex communities in unprecedented …
the field of ecology and allowed for the study of complex communities in unprecedented …
Polymerase matters: non-proofreading enzymes inflate fungal community richness estimates by up to 15%
Rare taxa overwhelm metabarcoding data generated using next-generation sequencing
(NGS). Low frequency Operational Taxonomic Units (OTUs) may be artifacts generated by …
(NGS). Low frequency Operational Taxonomic Units (OTUs) may be artifacts generated by …
Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?
True fungi (Fungi) and fungus-like organisms (eg Mycetozoa, Oomycota) constitute the
second largest group of organisms based on global richness estimates, with around 3 …
second largest group of organisms based on global richness estimates, with around 3 …
More bang for the buck? Can arbuscular mycorrhizal fungal communities be characterized adequately alongside other fungi using general fungal primers?
Background High throughput sequencing has revolutionized microbial ecology. We now
have the ability to characterize both prokaryotic and eukaryotic microbial communities more …
have the ability to characterize both prokaryotic and eukaryotic microbial communities more …
Different amplicon targets for sequencing-based studies of fungal diversity
Target-gene amplicon sequencing is the most exploited high-throughput sequencing
application in microbial ecology. The targets are taxonomically relevant genes, with 16S …
application in microbial ecology. The targets are taxonomically relevant genes, with 16S …
[PDF][PDF] Assessment of fungal diversity in the environment using metagenomics: a decade in review
S Cuadros-Orellana, LR Leite, A Smith… - Fungal Genomics & …, 2013 - researchgate.net
Fungi are ubiquitous inhabitants of soil and aquatic environments, and they establish and
maintain either parasitic or symbiotic relationships with animals and plants. They are major …
maintain either parasitic or symbiotic relationships with animals and plants. They are major …
[PDF][PDF] Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi
Rapid development of high-throughput (HTS) molecular identification methods has
revolutionized our knowledge about taxonomic diversity and ecology of fungi. However …
revolutionized our knowledge about taxonomic diversity and ecology of fungi. However …
Finding needles in haystacks: linking scientific names, reference specimens and molecular data for Fungi
DNA phylogenetic comparisons have shown that morphology-based species recognition
often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data …
often underestimates fungal diversity. Therefore, the need for accurate DNA sequence data …