Towards PacBio‐based pan‐eukaryote metabarcoding using full‐length ITS sequences
L Tedersoo, S Anslan - Environmental microbiology reports, 2019 - Wiley Online Library
Development of high‐throughput sequencing techniques has greatly benefited our
understanding about microbial ecology, yet the methods producing short reads suffer from …
understanding about microbial ecology, yet the methods producing short reads suffer from …
[HTML][HTML] Leveraging single-cell genomics to expand the fungal tree of life
Environmental DNA surveys reveal that most fungal diversity represents uncultured species.
We sequenced the genomes of eight uncultured species across the fungal tree of life using a …
We sequenced the genomes of eight uncultured species across the fungal tree of life using a …
[HTML][HTML] Extensive intragenomic variation in the internal transcribed spacer region of fungi
Fungi are among the most biodiverse organisms in the world. Accurate species identification
is imperative for studies on fungal ecology and evolution. The internal transcribed spacer …
is imperative for studies on fungal ecology and evolution. The internal transcribed spacer …
Sequence-based classification and identification of Fungi
Fungal taxonomy and ecology have been revolutionized by the application of molecular
methods and both have increasing connections to genomics and functional biology …
methods and both have increasing connections to genomics and functional biology …
[HTML][HTML] How, not if, is the question mycologists should be asking about DNA-based typification
Abstract Fungal metabarcoding of substrates such as soil, wood, and water is uncovering
an unprecedented number of fungal species that do not seem to produce tangible …
an unprecedented number of fungal species that do not seem to produce tangible …
Toward a fully resolved fungal tree of life
In this review, we discuss the current status and future challenges for fully elucidating the
fungal tree of life. In the last 15 years, advances in genomic technologies have …
fungal tree of life. In the last 15 years, advances in genomic technologies have …
PacBio metabarcoding of Fungi and other eukaryotes: errors, biases and perspectives
L Tedersoo, A Tooming‐Klunderud… - New Phytologist, 2018 - Wiley Online Library
Second‐generation, high‐throughput sequencing methods have greatly improved our
understanding of the ecology of soil microorganisms, yet the short barcodes (< 500 bp) …
understanding of the ecology of soil microorganisms, yet the short barcodes (< 500 bp) …
Sequencing our way towards understanding global eukaryotic biodiversity
Microscopic eukaryotes are abundant, diverse and fill critical ecological roles across every
ecosystem on Earth, yet there is a well-recognized gap in understanding of their global …
ecosystem on Earth, yet there is a well-recognized gap in understanding of their global …
Top 50 most wanted fungi
Environmental sequencing regularly recovers fungi that cannot be classified to any
meaningful taxonomic level beyond “Fungi”. There are several examples where evidence of …
meaningful taxonomic level beyond “Fungi”. There are several examples where evidence of …
Illumina metabarcoding of a soil fungal community
PA Schmidt, M Bálint, B Greshake, C Bandow… - Soil Biology and …, 2013 - Elsevier
Next generation metabarcoding is becoming an indispensable tool in fungal community
ecology. Here we tested Illumina metabarcoding, a method that generates shorter reads but …
ecology. Here we tested Illumina metabarcoding, a method that generates shorter reads but …