Ultra-deep sequencing enables high-fidelity recovery of biodiversity for bulk arthropod samples without PCR amplification
Background Next-generation-sequencing (NGS) technologies combined with a classic DNA
barcoding approach have enabled fast and credible measurement for biodiversity of mixed …
barcoding approach have enabled fast and credible measurement for biodiversity of mixed …
[图书][B] A practical guide to DNA-based methods for biodiversity assessment.
This book represents a synthesis of knowledge and best practice in the field of DNA-based
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …
Current insight into traditional and modern methods in fungal diversity estimates
Fungi are an important and diverse component in various ecosystems. The methods to
identify different fungi are an important step in any mycological study. Classical methods of …
identify different fungi are an important step in any mycological study. Classical methods of …
Metagenomics versus total RNA sequencing: most accurate data-processing tools, microbial identification accuracy and perspectives for ecological assessments
Metagenomics and total RNA sequencing (total RNA-Seq) have the potential to improve the
taxonomic identification of diverse microbial communities, which could allow for the …
taxonomic identification of diverse microbial communities, which could allow for the …
[HTML][HTML] Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding
Along with recent developments in high-throughput sequencing (HTS) technologies and
thus fast accumulation of HTS data, there has been a growing need and interest for …
thus fast accumulation of HTS data, there has been a growing need and interest for …
Historical biome distribution and recent human disturbance shape the diversity of arbuscular mycorrhizal fungi
The availability of global microbial diversity data, collected using standardized
metabarcoding techniques, makes microorganisms promising models for investigating the …
metabarcoding techniques, makes microorganisms promising models for investigating the …
Taxonomic reliability of DNA sequences in public sequence databases: a fungal perspective
Background DNA sequences are increasingly seen as one of the primary information
sources for species identification in many organism groups. Such approaches, popularly …
sources for species identification in many organism groups. Such approaches, popularly …
Parsing ecological signal from noise in next generation amplicon sequencing
It is clear that the use of next generation sequencing (NGS) applied to environmental DNA is
changing the way researchers conduct experiments and significantly deepening our …
changing the way researchers conduct experiments and significantly deepening our …
Evolutionary criteria outperform operational approaches in producing ecologically relevant fungal species inventories
Analyses of the structure and function of microbial communities are highly constrained by
the diversity of organisms present within most environmental samples. A common approach …
the diversity of organisms present within most environmental samples. A common approach …
FungalTraits vs. FUNGuild: comparison of ecological functional assignments of leaf-and needle-associated fungi across 12 temperate tree species
Recently, a new annotation tool “FungalTraits” was created based on the previous FUNGuild
and FunFun databases, which has attracted high attention in the scientific community. These …
and FunFun databases, which has attracted high attention in the scientific community. These …