Accurate digital polymerase chain reaction quantification of challenging samples applying inhibitor-tolerant DNA polymerases

M Sidstedt, EL Romsos, R Hedell, R Ansell… - Analytical …, 2017 - ACS Publications
Digital PCR (dPCR) enables absolute quantification of nucleic acids by partitioning of the
sample into hundreds or thousands of minute reactions. By assuming a Poisson distribution …

Digital polymerase chain reaction measured pUC19 marker as calibrant for HPLC measurement of DNA quantity

DG Burke, L Dong, S Bhat, M Forbes-Smith… - Analytical …, 2013 - ACS Publications
Digital polymerase chain reaction (dPCR) is potentially a primary method for quantifying
target DNA regions in a background of nontarget material and is independent of external …

Evaluating digital PCR for the quantification of human genomic DNA: accessible amplifiable targets

MC Kline, EL Romsos, DL Duewer - Analytical chemistry, 2016 - ACS Publications
Polymerase chain reaction (PCR) multiplexed assays perform best when the input quantity
of template DNA is controlled to within about a factor of√ 2. To help ensure that PCR assays …

Evaluation of digital PCR for absolute DNA quantification

R Sanders, JF Huggett, CA Bushell, S Cowen… - Analytical …, 2011 - ACS Publications
The emerging technique of microfluidic digital PCR (dPCR) offers a unique approach to real-
time quantitative PCR for measuring nucleic acids that may be particularly suited for low …

Basic concepts and validation of digital PCR measurements

L Pinheiro, KR Emslie - Digital PCR: Methods and Protocols, 2018 - Springer
Use of digital polymerase chain reaction (dPCR) technology is rapidly growing and
diversifying into a range of areas in life science. The release of dPCR commercial systems …

Quality control of digital PCR assays and platforms

M Vynck, J Vandesompele, O Thas - Analytical and bioanalytical chemistry, 2017 - Springer
Digital polymerase chain reaction (digital PCR, dPCR) is a direct nucleic acid quantification
method, thus requiring no standard curves unlike quantitative real-time PCR (qPCR) …

Real-time cdPCR opens a window into events occurring in the first few PCR amplification cycles

DL Duewer, MC Kline, EL Romsos - Analytical and bioanalytical chemistry, 2015 - Springer
Polymerase chain reaction (PCR) end-point limiting dilution techniques, collectively termed
“digital PCR (dPCR)”, have been proposed as providing a potentially primary method for …

Evaluating droplet digital polymerase chain reaction for the quantification of human genomic DNA: lifting the traceability fog

MC Kline, DL Duewer - Analytical chemistry, 2017 - ACS Publications
Digital polymerase chain reaction (dPCR) end point platforms directly estimate the number
of DNA target copies per reaction partition, λ, where the partitions are fixed-location …

[HTML][HTML] Medical diagnostic value of digital PCR (dPCR): A systematic review

S Nazir - Biomedical Engineering Advances, 2023 - Elsevier
Digital polymerase chain reaction (dPCR) is an emerging technique for the absolute
quantification of target nucleic acids. dPCR got attention as a precise quantification tool in …

Methods for applying accurate digital PCR analysis on low copy DNA samples

AS Whale, S Cowen, CA Foy, JF Huggett - PloS one, 2013 - journals.plos.org
Digital PCR (dPCR) is a highly accurate molecular approach, capable of precise
measurements, offering a number of unique opportunities. However, in its current format …