Three experiments were conducted in which 2-cell bovine embryos were prepared from
oocytes, obtained from abattoir ovaries, by in-vitro maturation for 22 to 24 hours, followed by …

Attempts to support survival of mammalian embryos after hatching have met with limited
success, although some mouse studies have reported growth at the post-implantation stage …

Currently, in vitro‐produced embryos derived by ovum pick up (OPU) and in vitro fertilization
(IVF) technologies represent approximately one‐third of the embryos worldwide in cattle …

It is well-established that in vitro culture affects quality, gene expression, and epigenetic
processes in bovine embryos and that trophectoderm cells are the most susceptible to …

Although toxic for early stages of embryo development, glucose is a physiological metabolic
substrate at the morula and blastocyst stages. We evaluated the effect of adding 5.5 mM …

Various factors such as embryo density and substances in the medium can influence
embryo development in vitro. These factors and the embryos probably interact with each …

The objective of this study was to determine the effects of fetal calf serum (FCS), non-
essential MEM amino acids, MEM vitamins and insulin on blastocoel formation, expansion …

A mammalian embryo originates from the fusion of a sperm cell with a mature oocyte. This
event usually takes place inside the maternal reproductive tract, but since the development …

Producing bovine in vitro embryos individually is a challenge as it generally leads to
impaired embryo development. Earlier research optimised a single embryo in vitro …

Recent advances in technologies associated with assisted reproduction in domestic animals
have been remarkable. In cattle, immature oocytes can be developed to the blastocyst stage …