MATERIALS AND METHODS Mononuclear cell [MNC) isolation. Human blood from healthy
donors at the local blood bank was collected in heparin. It was then diluted 1/3 in (penicillin …

MATERIALS AND METHODS Preparation of human lymphocyte populations: MN cells were
isolated from the peripheral blood of 16 healthy volunteers by Ficoll-Hypaque centrifugation …

Human peripheral blood mononuclear cells were separted into T and non-T cells by E-
rosette formation. The influence of histamine (10− 8− 10− 3 M) on the proliferative response …

The response of isolated T cells to autologous non-T mononuclear cells is called the
autologous mixed lymphocyte reaction (AMLR). The responding T cells show immunological …

The mixed lymphocyte reaction initially described by Bain et al.(1) and Hirschhorn et al.(2) is
currently being evaluated as a tool in many laboratories for histocompatibility testing …

We have prepared short term T cell lines, long term T cell lines from soft agar colonies, and
SMLR specific T cell hybridomas. Analysis of these populations has shown that the SMLR …

MATERIALS AND METHODS Cell preparation. Peripheral blood mononuclear cells (PBM)
were obtained from normal adult volunteers by centrifugation of heparinized venous blood …

Coculture of normal human T lymphocytes with autologous cells enriched in B lymphocytes
results in increased DNA synthesis. In our system, the responder cells are mononuclear …

We analyzed the effect of 2 hybridoma monoclonal antibodies (Mab), D1-12 and D4-22, with
specificity for common determinants of human Ia molecules, on the mixed lymphocyte …

The proliferative response of T cells to autologous non-T cells (autologous mixed leukocyte
reaction, AMLR) varies with the duration of incubation as well as the concentration and …