Small-molecule-induced protein depletion technologies, also called inducible degrons,
allow degradation of genetically engineered target proteins within cells and animals. Here …

Inducing post-translational protein knockdown is an important approach to probe biology
and validate drug targets. An efficient strategy to achieve this involves expression of a …

Dissection of complex biological systems requires target-specific control of the function or
abundance of proteins. Genetic perturbations are limited by off-target effects …

Chemical biology tools to modulate protein levels in cells are critical to decipher complex
biology. Targeted protein degradation offers the potential for rapid and dose-dependent …

Chemical biology strategies for directly perturbing protein homeostasis including the
degradation tag (dTAG) system provide temporal advantages over genetic approaches and …

To assess the role of a protein, protein loss phenotypic studies can be used, most commonly
through mutagenesis RNAi or CRISPR knockout. Such studies have been critical for the …

Conditional protein degradation tags (degrons) are usually> 100 amino acids long or are
triggered by small molecules with substantial off-target effects, thwarting their use as specific …

The ability to rapidly and selectively modulate cellular protein levels using small molecules
is essential for studying complex biological systems. Degradation tags, such as dTAG, allow …

Targeted protein degraders, known as proteolysis targeting chimeras (PROTACs), are
drawing more attention as next-generation drugs to target currently undruggable proteins …

The affinity-directed protein missile (AdPROM) system utilizes specific polypeptide binders
of intracellular proteins of interest (POIs) conjugated to an E3 ubiquitin ligase moiety to …