A direct look at RNAi screens

AR Barr, C Bakal - Molecular Systems Biology, 2012 - embopress.org
Over the last decade, cell-based RNAi screens have emerged as a powerful research tool.
High-content RNAi screening in particular has been successfully used to systematically …

Tales from an academic RNAi screening facility; FAQs

M Jiang, R Instrell, B Saunders… - Briefings in functional …, 2011 - academic.oup.com
RNAi technology is now a well-established and widely employed research technique that
has been adopted by many researchers for use in large-scale screening campaigns. Here …

An interactive web-based application for Comprehensive Analysis of RNAi-screen Data

B Dutta, A Azhir, LH Merino, Y Guo, S Revanur… - Nature …, 2016 - nature.com
RNAi screens are widely used in functional genomics. Although the screen data can be
susceptible to a number of experimental biases, many of these can be corrected by …

A genome-wide RNAi screening method to discover novel genes involved in virus infection

D Panda, S Cherry - Methods, 2015 - Elsevier
Systematic and comprehensive analysis of host cell proteins involved in virus infection has
been difficult in large part due to the lack of robust unbiased methods for their identification …

High-throughput RNAi screening in mammalian cells with esiRNAs

M Theis, F Buchholz - Methods, 2011 - Elsevier
The development of advanced functional genomic tools has paved the way for systematic
investigations of biological processes in health and disease. In particular, the …

Determination of sample size in genome-scale RNAi screens

XD Zhang, JF Heyse - Bioinformatics, 2009 - academic.oup.com
Abstract Motivation: For genome-scale RNAi research, it is critical to investigate sample size
required for the achievement of reasonably low false negative rate (FNR) and false positive …

Simultaneous analysis of large-scale RNAi screens for pathogen entry

P Rämö, A Drewek, C Arrieumerlou, N Beerenwinkel… - BMC genomics, 2014 - Springer
Abstract Background Large-scale RNAi screening has become an important technology for
identifying genes involved in biological processes of interest. However, the quality of large …

Cellular phenotyping by RNAi

F Fuchs, M Boutros - Briefings in Functional Genomics, 2006 - academic.oup.com
A systematic characterization of genes with unknown function is a key challenge after the
sequencing of the human genome and the genomes of many model organisms. High …

Genomic screening with RNAi: results and challenges

S Mohr, C Bakal, N Perrimon - Annual review of biochemistry, 2010 - annualreviews.org
RNA interference (RNAi) is an effective tool for genome-scale, high-throughput analysis of
gene function. In the past five years, a number of genome-scale RNAi high-throughput …

Systematic analysis of RNAi reports identifies dismal commonality at gene-level and reveals an unprecedented enrichment in pooled shRNA screens

B Bhinder, H Djaballah - Combinatorial chemistry & high …, 2013 - ingentaconnect.com
RNA interference (RNAi) has opened promising avenues to better understand gene
function. Though many RNAi screens report on the identification of genes, very few, if any …