A method for producing cloned pigs by using somatic cells as donors

L Lai, RS Prather - Germ Cell Protocols: Volume 2: Molecular Embryo …, 2004 - Springer
Nuclear transfer (NT) is a procedure by which genetically identical individuals can be
created. The applications of these NT techniques will be in agriculture, biomedicine, and …

Production of cloned pigs by using somatic cells as donors

L Lai, RS Prather - Cloning & Stem Cells, 2003 - liebertpub.com
NUCLEAR TRANSFER is a procedure by which genetically identical individuals can be
created. The applications of these techniques for nuclear transfer will be in agricultural …

Increasing efficiency in production of cloned piglets

H Callesen, Y Liu, HS Pedersen, R Li… - … (Formerly" Cloning and …, 2014 - liebertpub.com
The low efficiency in obtaining piglets after production of cloned embryos was challenged in
two steps—first by performing in vitro culture for 5–6 days after cloning to obtain later-stage …

Pig cloning using somatic cell nuclear transfer

H Ouyang, J Han, Y Huang - Nuclear Reprogramming: Methods and …, 2021 - Springer
Porcine cloning technology can be used to produce progenies genetically identical to the
donor cells from high-quality breeding pigs. In addition, genetically modified pigs have been …

Production of transgenic and knockout pigs by somatic cell nuclear transfer

AM Giraldo, S Ball, KR Bondioli - Xenotransplantation: Methods and …, 2012 - Springer
Xenotransplantation is one alternative to transplantation of human organs which has been
investigated. It is generally accepted that the pig represents the most logical choice of …

Nuclear transfer and transgenesis in the pig

M Kurome, B Kessler, A Wuensch… - … : Methods and Protocols, 2015 - Springer
Somatic cell nuclear transfer (SCNT) using genetically modified donor cells facilitates the
generation of tailored pig models for biomedical research and for xenotransplantation. Up to …

Cloning pigs: advances and applications.

IA Polejaeva - Reproduction (Cambridge, England). Supplement, 2001 - europepmc.org
Although mouse embryonic stem cells have been used widely for over a decade as an
important tool for introducing precise genetic modification into the genome, demonstrating …

Development and validation of a highly efficient protocol of porcine somatic cloning using preovulatory embryo transfer in peripubertal gilts

B Petersen, A Lucas-Hahn, M Oropeza… - Cloning and stem …, 2008 - liebertpub.com
The efficiency of porcine somatic nuclear transfer (born piglets/transferred embryos) is low.
Here, we report a highly efficient protocol using peripubertal gilts as recipients synchronized …

The use of nuclear transfer to produce transgenic pigs

Z Macháty, KR Bondioli, JJ Ramsoondar… - Cloning & Stem …, 2002 - liebertpub.com
Manipulation of the pig genome has the potential to improve pig production and offers
powerful biomedical applications. Genetic manipulation of mammals has been possible for …

Cloned pigs derived from somatic cell nuclear transfer embryos cultured in vitro at low oxygen tension

D Pan, Y Zhang, X Sun, J Zhang, X Li, Y Li, Z Gu… - Chinese Science …, 2006 - Springer
Pig cloning has great potential to human xenotransplantation. The present study was
designed to establish a more efficient system for producing cloned pigs by somatic cell …