Miniature pigs have been recognized as valuable experimental animals in various fields
such as medical and pharmaceutical research. However, the amount of information on …

Nuclear transfer (NT) is a procedure by which genetically identical individuals can be
created. The technology of pig somatic NT, including in vitro maturation of oocytes, isolation …

Different factors are believed to influence the outcome of nuclear transfer (NT) experiments.
Besides the cell cycle stage of both recipient cytoplast and donor karyoplast, the origin of the …

Details of the first mammal born after nuclear transfer cloning were published by Steen Malte
Willadsen in 1986. In spite of its enormous scientific significance, this discovery failed to …

Background Somatic cell nuclear transfer (SCNT) using genetically engineered donor cells
is currently the most widely used strategy to generate tailored pig models for biomedical …

Ruminants were the first mammalian species to be cloned successfully by nuclear
transplantation. Those experiments were designed to multiply high merit animals (Willadsen …

Currently, cloning efficiency in pigs is very low. Donor cell type and number of cloned
embryos transferred to an individual surrogate are two major factors that affect the …

The production of animals by somatic cell nuclear transfer (SCNT) is inefficient, with
approximately 2% of micromanipulated oocytes going to term and resulting in live births …

THE 10TH ANNIVERSARY of the report of the first animal cloned from an adult cell (Wilmut
et al., 1997) is an excellent time to look back, and to look forward. The ability to clone …

Puromycin N-acetyl transferase gene (pac), of which the gene product catalyzes antibiotic
puromycin (an effective inhibitor of protein synthesis), has been widely used as a dominant …