The guideline of the design and validation of MiRNA mimics
Z Wang - MicroRNA and Cancer: Methods and Protocols, 2011 - Springer
The miRNA mimic technology (miR-Mimic) is an innovative approach for gene silencing.
This approach is to generate nonnatural double-stranded miRNA-like RNA fragments. Such …
This approach is to generate nonnatural double-stranded miRNA-like RNA fragments. Such …
[HTML][HTML] Effective tools for RNA-derived therapeutics: siRNA interference or miRNA mimicry
P Wang, Y Zhou, AM Richards - Theranostics, 2021 - ncbi.nlm.nih.gov
The approval of the first small interfering RNA (siRNA) drug Patisiran by FDA in 2018 marks
a new era of RNA interference (RNAi) therapeutics. MicroRNAs (miRNA), an important post …
a new era of RNA interference (RNAi) therapeutics. MicroRNAs (miRNA), an important post …
Transcriptional gene silencing in mammalian cells by miRNA mimics that target gene promoters
ST Younger, DR Corey - Nucleic acids research, 2011 - academic.oup.com
Synthetic small duplex RNAs that are fully complementary to gene promoters can silence
transcription in mammalian cells. microRNAs (miRNAs) are endogenous small regulatory …
transcription in mammalian cells. microRNAs (miRNAs) are endogenous small regulatory …
Transfection of microRNA mimics should be used with caution
HY Jin, A Gonzalez-Martin, AV Miletic, M Lai… - Frontiers in …, 2015 - frontiersin.org
Transient transfection of chemically synthesized microRNA (miRNA) mimics is being used
extensively to study the functions and mechanisms of endogenous miRNAs. However, it …
extensively to study the functions and mechanisms of endogenous miRNAs. However, it …
Reexamining assumptions about miRNA-guided gene silencing
MicroRNAs (miRNAs) are short endogenously expressed RNAs that have the potential to
regulate the expression of any RNA. This potential has led to the publication of several …
regulate the expression of any RNA. This potential has led to the publication of several …
[图书][B] miRNA Interference Technologies: An Overview
Z Wang, Z Wang - 2009 - Springer
The miRNA pathways are highly responsive to interventions of any kind, being excellent
candidates for pharmacological manipulation. Mature miR-NAs belong to a category of non …
candidates for pharmacological manipulation. Mature miR-NAs belong to a category of non …
Exploiting and antagonizing microRNA regulation for therapeutic and experimental applications
BD Brown, L Naldini - Nature Reviews Genetics, 2009 - nature.com
New technologies are emerging that utilize artificial microRNA (miRNA) target sites to exploit
or inhibit endogenous miRNA regulation. This approach has been used to improve cell …
or inhibit endogenous miRNA regulation. This approach has been used to improve cell …
Passenger strand loading in overexpression experiments using microRNA mimics
MicroRNAs (miRNAs) are important regulators of gene function and manipulation of miRNAs
is a central component of basic research. Modulation of gene expression by miRNA gain-of …
is a central component of basic research. Modulation of gene expression by miRNA gain-of …
Dual luciferase gene reporter assays to study miRNA function
T Clément, V Salone, M Rederstorff - Small non-coding RNAs: methods …, 2015 - Springer
This chapter describes one of the most reliable quantitative assays to test the silencing of a
possible target gene by a specific miRNA using a luciferase reporter gene. The experimental …
possible target gene by a specific miRNA using a luciferase reporter gene. The experimental …
miRNA cassettes in viral vectors: problems and solutions
YP Liu, B Berkhout - Biochimica et Biophysica Acta (BBA)-Gene Regulatory …, 2011 - Elsevier
The discovery of RNA interference (RNAi), an evolutionary conserved gene silencing
mechanism that is triggered by double stranded RNA, has led to tremendous efforts to use …
mechanism that is triggered by double stranded RNA, has led to tremendous efforts to use …