Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated
(Cas) systems have evolved in bacteria and archaea as a defense mechanism to silence …

CRISPR-Cas systems enable microbial adaptive immunity and provide eukaryotic genome
editing tools. These tools employ a single effector enzyme of type II or V CRISPR to generate …

Targeted nucleases are powerful tools for mediating genome alteration with high precision.
The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short …

CRISPR/Cas complexes enable precise gene editing in a wide variety of organisms. While
the rigid identification of DNA sequences by these systems minimizes the potential for off …

Bacterial CRISPR–Cas systems comprise diverse effector endonucleases with different
targeting ranges, specificities and enzymatic properties, but many of them are inactive in …

CRISPR/Cas is a microbial adaptive immune system that uses RNA-guided nucleases to
cleave foreign genetic elements. The CRISPR/Cas9 method has been engineered from the …

A key limitation of the use of the CRISPR–Cas9 system for genome editing and other
applications is the requirement that a protospacer adjacent motif (PAM) be present at the …

CRISPR-Cas genome engineering has revolutionized biomedical research by enabling
targeted genome modification with unprecedented ease. In the popular model organism …

Abstract Type I CRISPR–Cas systems are the most abundant adaptive immune systems in
bacteria and archaea,. Target interference relies on a multi-subunit, RNA-guided complex …

Targeted genome editing using engineered nucleases has rapidly gone from being a niche
technology to a mainstream method used by many biological researchers. This widespread …