The hypo-osmotic swelling test for evaluation of sperm membrane integrity

S Ramu, RS Jeyendran - Spermatogenesis: Methods and protocols, 2013 - Springer
A functional membrane is requisite for the fertilizing ability of spermatozoa, as it plays an
integral role in sperm capacitation, acrosome reaction, and binding of the spermatozoon to …

The relationship between the human sperm hypoosmotic swelling test, routine semen analysis, and the human sperm zona-free hamster ovum penetration assay

SYW Chan, EJ Fox, MMC Chan, W Tsoi, C Wang… - Fertility and sterility, 1985 - Elsevier
The functional integrity of sperm membranes of 270 semen samples collected from fertile
men and the male partners in couples with infertile marriages was assessed by the …

Adaptation of the hypoosmotic swelling test to assess functional integrity of stallion spermatozoal plasma membranes

GJ Nie, JGW Wenzel - Theriogenology, 2001 - Elsevier
Hypoosmotic swelling (HOS) is used for assessing plasma membrane function and fertilizing
capacity of human spermatozoa. However, HOS solutions and methodologies have not …

Correlation Between Human Sperm Swelling in Hypoosmotic Medium (Hypoosmotic Swelling Test) and In Vitro Fertilization

HH VAN DER VEN, RS JEYENDRAN… - Journal of …, 1986 - Wiley Online Library
Human ejaculates (n= 83) were analyzed for standard sperm parameters (concentration,
motility, and morphology), as well as for the ability of the spermatozoa to react (swell) in a …

Effects of hypoosmotic incubation on acrosome and tail structure on canine spermatozoa

JE Rodriguez-Gil, A Montserrat, T Rigau - Theriogenology, 1994 - Elsevier
Hypoosmotic tests are widely used as valuable tests for determining sperm quality in
species as varied as the human and the porcine. However, there is little information about …

Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa

HD Guthrie, J Liu, JK Critser - Biology of reproduction, 2002 - academic.oup.com
This study was conducted to determine the osmotic properties of bull spermatozoa, including
the effects of osmotic stress and cryoprotectant agent (CPA) addition and removal, on sperm …

A short hypoosmotic swelling test for the prediction of boar sperm fertility

B Pérez-Llano, JL Lorenzo, P Yenes, A Trejo… - Theriogenology, 2001 - Elsevier
This study was designed to explore the relationship between the ejaculate response to a
hypoosmotic swelling test (HOST) and in vivo fertility in a group of 38 boars. The …

Prognostic value of spermatological parameters as predictors of in vitro fertility of frozen-thawed bull semen

CM Tartaglione, MN Ritta - Theriogenology, 2004 - Elsevier
Cryopreservation imposes irreversible damage to sperm membranes, such as swelling and
disruption of plasma and acrosome membranes, changes in membrane fluidity, altered …

Comparison of different hypo-osmotic swelling solutions to select viable immotile spermatozoa for potential use in intracytoplasmic sperm injection

G Verheyen, H Joris, K Crits, Z Nagy… - Human reproduction …, 1997 - academic.oup.com
The hypo-osmotic swelling test, originally developed as a diagnostic sperm test, is used to
discriminate viable from non-viable spermatozoa for intracytoplasmic sperm injection (ICSI) …

Hyperosmotic tolerance of human spermatozoa: separate effects of glycerol, sodium chloride, and sucrose on spermolysis

DY Gao, E Ashworth, PF Watson… - Biology of …, 1993 - academic.oup.com
Hyperosmotic stress, which cells experience during the freezing process, and its release
during the warming process are both related to cryoinjury. To define optimal cooling or …