In preparation for transfer conjugative type IV secretion systems (T4SS) produce a
nucleoprotein adduct containing a relaxase enzyme covalently linked to the 5′ end of …

Type IV secretion system (T4SS) substrates are recruited through a translocation signal that
is poorly defined for conjugative relaxases. The relaxase TrwC of plasmid R388 is …

Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from
one bacterium to another during conjugation. They covalently react with a specific …

Bacterial conjugation, a mechanism of horizontal gene transfer, is the major means by which
antibiotic resistance spreads among bacteria (1, 2). Conjugative plasmids are transferred …

Bacteria commonly exchange genetic information by the horizontal transfer of conjugative
plasmids. In gram-negative conjugation, a relaxase enzyme is absolutely required to …

A large subfamily of the type IV secretion systems (T4SSs), termed the conjugation systems,
transmit mobile genetic elements (MGEs) among many bacterial species. In the initiating …

All plasmids that spread by conjugative transfer encode a relaxase. That includes plasmids
that encode the type IV secretion machinery necessary to mediate cell to cell transfer, as …

Conjugation, the process by which plasmid DNA is transferred from one bacterium to
another, is mediated by type IV secretion systems (T4SSs). T4SSs are versatile systems that …

We isolated and characterized traD mutants with an altered specificity of interaction with
relaxosomes of various conjugative (F and R388) and mobilizable (RSF1010 and ColE1) …

Conjugative plasmid transfer is an important mechanism for diversifying prokaryotic
genomes and disseminating antibiotic resistance. Relaxases are conjugative plasmid …