Precise gene editing in human pluripotent stem cells (hPSCs) holds great promise for
studying and potentially treating human diseases. Both prime editing and base editing avoid …

The recent development of prime editing (PE) genome engineering technologies has the
potential to significantly simplify the generation of human pluripotent stem cell (hPSC)-based …

Human pluripotent stem cells (hPSCs) offer unprecedented opportunities to study cellular
differentiation and model human diseases. The ability to precisely modify any genomic …

Genome editing in induced pluripotent stem cells is currently hampered by the laborious and
expensive nature of identifying homology-directed repair (HDR)-modified cells. We present …

The utility of genome editing technologies for disease modeling and developing cellular
therapies has been extensively documented, but the impact of these technologies on …

I report here a detailed protocol for seamless genome editing using the piggyBac
transposon in human pluripotent stem cells (hPSCs). Recent advances in custom …

The advent of induced pluripotent stem (iPS) cells has opened up numerous avenues of
opportunity for cell therapy, including the initiation in September 2014 of the first human …

In the decade since Yamanaka and colleagues described methods to reprogram somatic
cells into a pluripotent state, human induced pluripotent stem cells (hiPSCs) have …

Precise editing of human genomes in pluripotent stem cells by homology-driven repair of
targeted nuclease–induced cleavage has been hindered by the difficulty of isolating rare …

Human pluripotent stem cells (hPSCs) are a powerful platform for disease modeling and
drug discovery. However, the introduction of known pathogenic mutations into hPSCs is a …