Brain organ cells were cultured on cellulose polyacetate and other substrates in order to
investigate metastatic tumor cell invasion in vitro. The cultures consisted of small pieces …

Purpose. The primary cause of local recurrence and therapeutic failure in the treatment of
malignant gliomas is the invasion of tumor cells into the surrounding normal brain. While it is …

The present knowledge about the interaction between the extracellular matrix (ECM) and
gliomas is mostly based on studies of permanent cell lines. Since such cultures have …

Background Multicellular spheroids, an appropriate in vitro system for simulating 3-D tumor
micro-milieu can be used for evaluating and predicting tumor response to therapeutic agents …

Multicellular tumour spheroids were prepared from a total of 46 human brain tumour
biopsies by collagenase digestion and plating into agar coated flasks. Both primary …

The multicellular tumor spheroid model is a widely used culture system to mimic the three-
dimensionality of tumors. Several methods and an even larger number of protocols are …

Spheroids are widely used in biology because they provide an in vitro 3-dimensional (3D)
model to study proliferation, cell death, differentiation, and metabolism of cells in tumors and …

Many types of mammalian cells can aggregate and differentiate into 3‐D multicellular
spheroids when cultured in suspension or a nonadhesive environment. Compared to …

Tumour spheroids initiated from glioma biopsy specimens provide a valuable three‐
dimensional cell culture system that share several biological features of malignant brain …

Sphere forming assays have been useful to enrich for stem like cells in a range of tumors.
The robustness of this system contrasts the difficulties in defining a stem cell population …