Monolayer cell culture does not adequately model the in vivo behavior of tissues, which
involves complex cell-cell and cell-matrix interactions. Three-dimensional (3D) cell culture …

Threedimensional culture systems, pioneered with the development by Moscona (1961) of
the technology to culture embryonic cells in reaggregate structures and by Sutherland and …

Multicellular tumour spheroids (MCTS), the best-described three-dimensional tumour model,
offers an excellent in vitro screening system, which to a great extent mimics the …

Invasion of surrounding normal tissues is generally considered to be a key hallmark of
malignant (as opposed to benign) tumors. For some cancers in particular (eg, brain tumors …

In vitro 3D cancer spheroids (tumoroids) exhibit a drug resistance profile similar to that found
in solid tumors. 3D spheroid culture methods recreate more physiologically relevant …

Major Glioblastoma's hallmarks include proliferation, invasion and heterogeneity. Biological
3D tumor spheroid models can serve as intermediate systems between traditional 2D cell …

Malignant growth of cells is often characterized by disorganization of tissue structure,
abnormal blood vessel development, and insuffi cient vascular supply. As a consequence …

The brain tumor stem cell (BTSC) hypothesis is based on the premise that there is a
subpopulation of cells within tumors with tumorigenic and pluripotent properties. BTSC are …

Certain limitations of the neurosphere assay (NSA) have resulted in a search for alternative
culture techniques for brain tumor-initiating cells (TICs). Recently, reports have described …

The present article highlights the rationale, potential and flexibility of tumor spheroid mono-
and cocultures for implementation into state of the art anti-cancer therapy test platforms …