Construction and characterization of a human bacterial artificial chromosome library

UJ Kim, BW Birren, T Slepak, V Mancino, C Boysen… - Genomics, 1996 - Elsevier
We have constructed an arrayed human genomic BAC library with approximately 4×
coverage that is represented by 96,000 BAC clones with average insert size of nearly 140 …

Human BAC library: construction and rapid screening

S Asakawa, I Abe, Y Kudoh, N Kishi, Y Wang, R Kubota… - Gene, 1997 - Elsevier
We have constructed a human genomic bacterial artificial chromosome (BAC) library using
high molecular weight DNA from a pre-pro-B cell line, FLEB14-14, with a normal male …

Bacterial artificial chromosome libraries for mouse sequencing and functional analysis

K Osoegawa, M Tateno, PY Woon, E Frengen… - Genome …, 2000 - genome.cshlp.org
Bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC) libraries
providing a combined 33-fold representation of the murine genome have been constructed …

A bacterial artificial chromosome library for sequencing the complete human genome

K Osoegawa, AG Mammoser, C Wu, E Frengen… - Genome …, 2001 - genome.cshlp.org
A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large
average insert size (178 kb) has been constructed to provide the intermediate substrate for …

A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites

E Frengen, D Weichenhan, B Zhao, K Osoegawa… - Genomics, 1999 - Elsevier
To construct large-insert libraries for the sequencing, mapping, and functional studies of
complex genomes, we have constructed a new modular bacterial artificial chromosome …

High throughput direct end sequencing of BAC clones

JM Kelley, CE Field, M Brook Craven… - Nucleic Acids …, 1999 - academic.oup.com
Libraries constructed in bacterial artificial chromosome (BAC) vectors have become the
choice for clone sets in high throughput genomic sequencing projects primarily because of …

Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library

R Li, E Mignot, J Faraco, H Kadotani, J Cantanese… - Genomics, 1999 - Elsevier
A large insert canine genomic bacterial artificial chromosome (BAC) library was built from a
Doberman pinscher. Approximately 166,000 clones were gridded on nine high-density …

An improved approach for construction of bacterial artificial chromosome libraries

K Osoegawa, PY Woon, B Zhao, E Frengen, M Tateno… - Genomics, 1998 - Elsevier
Presented here are improved methodologies that enable the generation of highly redundant
bacterial artificial chromosome/P1-derived artificial chromosome libraries, with larger and …

Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system

K Narayanan, R Williamson, Y Zhang, AF Stewart… - Gene therapy, 1999 - nature.com
Gene therapy studies require techniques that allow alteration of human genomic DNA
sequences. Bacterial artificial chromosome cloning systems (BACs/PACs) bridge the gap …

The development and applications of the bacterial artificial chromosome cloning system

H Shizuya, H Kouros-Mehr - The Keio journal of medicine, 2001 - jstage.jst.go.jp
The development of the Bacterial Artificial Chromosome (BAC) system was driven in part by
the Human Genome Project as a means to construct genomic DNA libraries and physical …