Construction and characterization of a human bacterial artificial chromosome library
UJ Kim, BW Birren, T Slepak, V Mancino, C Boysen… - Genomics, 1996 - Elsevier
We have constructed an arrayed human genomic BAC library with approximately 4×
coverage that is represented by 96,000 BAC clones with average insert size of nearly 140 …
coverage that is represented by 96,000 BAC clones with average insert size of nearly 140 …
Human BAC library: construction and rapid screening
S Asakawa, I Abe, Y Kudoh, N Kishi, Y Wang, R Kubota… - Gene, 1997 - Elsevier
We have constructed a human genomic bacterial artificial chromosome (BAC) library using
high molecular weight DNA from a pre-pro-B cell line, FLEB14-14, with a normal male …
high molecular weight DNA from a pre-pro-B cell line, FLEB14-14, with a normal male …
Bacterial artificial chromosome libraries for mouse sequencing and functional analysis
K Osoegawa, M Tateno, PY Woon, E Frengen… - Genome …, 2000 - genome.cshlp.org
Bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC) libraries
providing a combined 33-fold representation of the murine genome have been constructed …
providing a combined 33-fold representation of the murine genome have been constructed …
A bacterial artificial chromosome library for sequencing the complete human genome
K Osoegawa, AG Mammoser, C Wu, E Frengen… - Genome …, 2001 - genome.cshlp.org
A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large
average insert size (178 kb) has been constructed to provide the intermediate substrate for …
average insert size (178 kb) has been constructed to provide the intermediate substrate for …
A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites
E Frengen, D Weichenhan, B Zhao, K Osoegawa… - Genomics, 1999 - Elsevier
To construct large-insert libraries for the sequencing, mapping, and functional studies of
complex genomes, we have constructed a new modular bacterial artificial chromosome …
complex genomes, we have constructed a new modular bacterial artificial chromosome …
High throughput direct end sequencing of BAC clones
JM Kelley, CE Field, M Brook Craven… - Nucleic Acids …, 1999 - academic.oup.com
Libraries constructed in bacterial artificial chromosome (BAC) vectors have become the
choice for clone sets in high throughput genomic sequencing projects primarily because of …
choice for clone sets in high throughput genomic sequencing projects primarily because of …
Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library
A large insert canine genomic bacterial artificial chromosome (BAC) library was built from a
Doberman pinscher. Approximately 166,000 clones were gridded on nine high-density …
Doberman pinscher. Approximately 166,000 clones were gridded on nine high-density …
An improved approach for construction of bacterial artificial chromosome libraries
K Osoegawa, PY Woon, B Zhao, E Frengen, M Tateno… - Genomics, 1998 - Elsevier
Presented here are improved methodologies that enable the generation of highly redundant
bacterial artificial chromosome/P1-derived artificial chromosome libraries, with larger and …
bacterial artificial chromosome/P1-derived artificial chromosome libraries, with larger and …
Efficient and precise engineering of a 200 kb β-globin human/bacterial artificial chromosome in E. coli DH10B using an inducible homologous recombination system
Gene therapy studies require techniques that allow alteration of human genomic DNA
sequences. Bacterial artificial chromosome cloning systems (BACs/PACs) bridge the gap …
sequences. Bacterial artificial chromosome cloning systems (BACs/PACs) bridge the gap …
The development and applications of the bacterial artificial chromosome cloning system
H Shizuya, H Kouros-Mehr - The Keio journal of medicine, 2001 - jstage.jst.go.jp
The development of the Bacterial Artificial Chromosome (BAC) system was driven in part by
the Human Genome Project as a means to construct genomic DNA libraries and physical …
the Human Genome Project as a means to construct genomic DNA libraries and physical …