Human papillomavirus testing methods

RA Hubbard - Archives of pathology & laboratory medicine, 2003 - meridian.allenpress.com
Testing for human papillomavirus (HPV) relies exclusively on techniques of molecular
biology using nucleic acid probes. Tests for HPV using nucleic acid probes have been …

Comparison of human papillomavirus detection and typing by cycle sequencing, line blotting, and hybrid capture

SD Vernon, ER Unger, D Williams - Journal of clinical …, 2000 - Am Soc Microbiol
We compared the results of human papillomavirus (HPV) detection and typing from 781
cervical samples assayed by three methods: L1 consensus PCR followed by cycle …

Genotyping of 27 human papillomavirus types by using L1 consensus PCR products by a single-hybridization, reverse line blot detection method

PE Gravitt, CL Peyton, RJ Apple… - Journal of clinical …, 1998 - Am Soc Microbiol
Amplification of human papillomavirus (HPV) DNA by L1 consensus primer systems (eg,
MY09/11 or GP5+/6+) can detect as few as 10 to 100 molecules of HPV targets from a …

Comparison of real-time multiplex human papillomavirus (HPV) PCR assays with the linear array HPV genotyping PCR assay and influence of DNA extraction method …

CC Roberts, R Swoyer, JT Bryan… - Journal of clinical …, 2011 - Am Soc Microbiol
Real-time human papillomavirus (HPV) type-specific multiplex PCR assays were developed
to detect HPV DNA in specimens collected for the efficacy determination of the quadrivalent …

Chapter 12: Human papillomavirus technologies

T Iftner, LL Villa - JNCI Monographs, 2003 - academic.oup.com
Currently, human papillomavirus (HPV) DNA tests validated in large trials and
epidemiological studies are the hybrid capture second-generation (HC2) HPV DNA assay …

Typing of human papillomaviruses by polymerase chain reaction amplification with L1 consensus primers and RFLP analysis

O Lungu, TC Wright Jr, S Silverstein - Molecular and cellular Probes, 1992 - Elsevier
Human papillomaviruses (HPV) cause benign and malignant lesions of the epithelial and
mucosal surfaces. Certain virus types are associated with cervical carcinomas, 1–3 while …

Bead-based multiplex genotyping of human papillomaviruses

M Schmitt, IG Bravo, PJF Snijders… - Journal of clinical …, 2006 - Am Soc Microbiol
Typing of human papillomaviruses (HPV) by DNA hybridization procedures, such as reverse
line blot (RLB) assay, is sensitive and well validated. However, the application of these …

Polymerase chain reaction-based methods for the detection of human papillomavirus DNA.

PE Gravitt, MM Manos - IARC scientific publications, 1992 - europepmc.org
The unprecedented sensitivity of the polymerase chain reaction (PCR) DNA amplification
technology provides numerous advantages for the detection of human papillomavirus (HPV) …

A novel strategy for human papillomavirus detection and genotyping with SybrGreen and molecular beacon polymerase chain reaction

K Szuhai, E Sandhaus, SM Kolkman-Uljee… - The American journal of …, 2001 - Elsevier
Human papillomaviruses (HPVs) play an important role in the pathogenesis of cervical
cancer. For identification of the large number of different HPV types found in (pre) malignant …

High-throughput detection, genotyping and quantification of the human papillomavirus using real-time PCR

IM Micalessi, GAV Boulet, JJ Bogers… - Clinical chemistry and …, 2012 - degruyter.com
Background: The establishment of the causal relationship between high-risk human
papillomavirus (HR-HPV) infection and cervical cancer and its precursors has resulted in the …