An important reason for preferring mammalian cells for heterologous gene expression is
their ability to make authentic proteins containing post‐translational modifications similar to …

The increasing knowledge of the molecular and genetic background of many different
human diseases has led to the vision that genetic engineering might be used one day for …

The transfer of foreign genes into eukaryotic cells, in particular mammalian cells, has been
essential to our understanding of the functional significance of genes and regulatory …

For long-term gene expression in tissues, we constructed an Epstein–Barr virus (EBV)
replicon-based plasmid, pEB, containing the latent viral DNA replication origin (oriP) and …

Progress in diverse scientific fields has been realized partly by the continued refinement of
mammalian gene expression vectors. A growing understanding of biological processes now …

Mammalian expression systems have an undisputed long-standing and very successful
history for the generation of recombinant proteins, mainly as biopharmaceuticals. However …

The expression of cloned genes in mammalian cells is a basic tool for understanding gene
expression, protein structure, and function, and biological regulatory mechanisms. The level …

The transient expression level of foreign proteins in primate cells can be enhanced by
incorporating the replication elements derived from Epstein-Barr virus (EBV). Specifically …

Efficient transfection and expression of cDNA libraries in human cells has been achieved
with an Epstein-Barr virus-based subcloning vector (EBO-pcD). The plasmid vector contains …

Various methods have been developed to transfer and express genes in mammalian cells.
Each method, whether virally, non-virally, or physically-based, has unique favorable …