Relative performance of customized and universal probe sets in target enrichment: A case study in subtribe Malinae

R Ufimov, V Zeisek, S Píšová, WJ Baker… - Applications in Plant …, 2021 - Wiley Online Library
Premise Custom probe design for target enrichment in phylogenetics is tedious and often
hinders broader phylogenetic synthesis. The universal angiosperm probe set …

New targets acquired: Improving locus recovery from the Angiosperms353 probe set

TGB McLay, JL Birch, BF Gunn, W Ning… - Applications in plant …, 2021 - Wiley Online Library
PREMISE Universal target enrichment kits maximize utility across wide evolutionary breadth
while minimizing the number of baits required to create a cost‐efficient kit. The …

Utilizing paralogues for phylogenetic reconstruction has the potential to increase species tree support and reduce gene tree discordance in target enrichment data

R Ufimov, JM Gorospe, T Fér… - Molecular Ecology …, 2022 - Wiley Online Library
The analysis of target enrichment data in phylogenetics lacks optimization toward using
paralogues for phylogenetic reconstruction. We developed a novel approach of detecting …

A universal probe set for targeted sequencing of 353 nuclear genes from any flowering plant designed using k-medoids clustering

MG Johnson, L Pokorny, S Dodsworth… - Systematic …, 2019 - academic.oup.com
Sequencing of target-enriched libraries is an efficient and cost-effective method for obtaining
DNA sequence data from hundreds of nuclear loci for phylogeny reconstruction. Much of the …

A hybrid capture RNA bait set for resolving genetic and evolutionary relationships in angiosperms from deep phylogeny to intraspecific lineage hybridization

M Waycott, K van Dijk, E Biffin - BioRxiv, 2021 - biorxiv.org
Novel multi-gene targeted capture probes have been developed with the objective of
obtaining multi-locus high quality sequence reads across any angiosperm lineage. Using …

Easy353: A tool to get Angiosperms353 genes for phylogenomic research

Z Zhang, P Xie, Y Guo, W Zhou, E Liu… - Molecular Biology and …, 2022 - academic.oup.com
The Angiosperms353 gene set (AGS) consists of a set of 353 universal low-copy nuclear
genes that were selected by examining more than 600 angiosperm species. These genes …

A new pipeline for removing paralogs in target enrichment data

W Zhou, J Soghigian, QY Xiang - Systematic Biology, 2022 - academic.oup.com
Target enrichment (such as Hyb-Seq) is a well-established high throughput sequencing
method that has been increasingly used for phylogenomic studies. Unfortunately, current …

On the potential of Angiosperms353 for population genomic studies

M Slimp, LD Williams, H Hale… - Applications in Plant …, 2021 - Wiley Online Library
PREMISE The successful application of universal targeted sequencing markers, such as
those developed for the Angiosperms353 probe set, within populations could reduce or …

The best of both worlds: Combining lineage‐specific and universal bait sets in target‐enrichment hybridization reactions

KP Hendriks, T Mandáková, NM Hay… - Applications in Plant …, 2021 - Wiley Online Library
Premise Researchers adopting target‐enrichment approaches often struggle with the
decision of whether to use universal or lineage‐specific probe sets. To circumvent this …

Anchored phylogenomics of angiosperms I: assessing the robustness of phylogenetic estimates

C Buddenhagen, AR Lemmon, EM Lemmon, J Bruhl… - BioRxiv, 2016 - biorxiv.org
An important goal of the angiosperm systematics community has been to develop a shared
approach to molecular data collection, such that phylogenomic data sets from different focal …