Reconstitution of membrane proteins into polymer-supported membranes for probing diffusion and interactions by single molecule techniques

F Roder, S Waichman, D Paterok, R Schubert… - Analytical …, 2011 - ACS Publications
We have established a robust and versatile analytical platform for probing membrane
protein function in a defined lipid environment on solid supports. This approach is based on …

Diffusion and Interaction Dynamics of Individual Membrane Protein Complexes Confined in Micropatterned Polymer‐Supported Membranes

S Waichman, F Roder, CP Richter, O Birkholz, J Piehler - Small, 2013 - Wiley Online Library
Micropatterned polymer‐supported membranes (PSM) are established as a tool for
confining the diffusion of transmembrane proteins for single molecule studies. To this end, a …

Proteins on supported lipid bilayers diffusing around proteins fixed on acrylate anchors

B Buchegger, J Kreutzer, M Axmann, S Mayr… - Analytical …, 2018 - ACS Publications
Mobility of proteins and lipids plays a major role in physiological processes. Platforms which
were developed to study protein interaction between immobilized and mobile proteins suffer …

Engineering Cell Membranes: From Extraction Strategies to Emerging Biosensing Applications

J Tan, C Zhu, L Li, J Wang, XH Xia, C Wang - Analytical Chemistry, 2024 - ACS Publications
The cell membrane, formed by the self-organization of phospholipids and various
biomacromolecules, is a biocomplex with the thickness of 5− 10 nm. 1− 3 Proteins bind lipid …

Lipid mobility and molecular binding in fluid lipid membranes

V Yamazaki, O Sirenko, RJ Schafer… - Journal of the American …, 2005 - ACS Publications
The lateral mobility of dilute concentrations of fluorescently labeled lipids doped into
supported membranes is found to change upon receptor ligand binding at the membrane …

Advances in Membrane Mimetic Systems for Manipulation and Analysis of Membrane Proteins: Detergents, Polymers, Lipids and Scaffolds

M Woubshete, S Cioccolo, B Byrne - ChemPlusChem, 2024 - Wiley Online Library
Extracting membrane proteins from the hydrophobic environment of the biological
membrane, in a physiologically relevant and stable state, suitable for downstream analysis …

Preserved transmembrane protein mobility in polymer-supported lipid bilayers derived from cell membranes

H Pace, L Simonsson Nyström… - Analytical …, 2015 - ACS Publications
Supported lipid bilayers (SLBs) have contributed invaluable information about the
physiochemical properties of cell membranes, but their compositional simplicity often limits …

[HTML][HTML] A strategic approach for fluorescence imaging of membrane proteins in a native-like environment

JM Swiecicki, JT Santana, B Imperiali - Cell chemical biology, 2020 - cell.com
Biological membranes are complex barriers in which membrane proteins and thousands of
lipidic species participate in structural and functional interactions. Developing a strategic …

Single-molecule resolution of protein dynamics on polymeric membrane surfaces: The roles of spatial and population heterogeneity

BB Langdon, RB Mirhossaini, JN Mabry… - … Applied Materials & …, 2015 - ACS Publications
Although polymeric membranes are widely used in the purification of protein
pharmaceuticals, interactions between biomolecules and membrane surfaces can lead to …

Characterization of single-protein dynamics in polymer-cushioned lipid bilayers derived from cell plasma membranes

WC Wong, JY Juo, CH Lin, YH Liao… - The Journal of …, 2019 - ACS Publications
Native cell-membrane-derived supported lipid bilayers (SLBs) are an emerging platform with
broad applications ranging from fundamental research to next-generation biosensors …