Bacterial artificial chromosome libraries for mouse sequencing and functional analysis

K Osoegawa, M Tateno, PY Woon, E Frengen… - Genome …, 2000 - genome.cshlp.org
Bacterial artificial chromosome (BAC) and P1-derived artificial chromosome (PAC) libraries
providing a combined 33-fold representation of the murine genome have been constructed …

An improved approach for construction of bacterial artificial chromosome libraries

K Osoegawa, PY Woon, B Zhao, E Frengen, M Tateno… - Genomics, 1998 - Elsevier
Presented here are improved methodologies that enable the generation of highly redundant
bacterial artificial chromosome/P1-derived artificial chromosome libraries, with larger and …

A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites

E Frengen, D Weichenhan, B Zhao, K Osoegawa… - Genomics, 1999 - Elsevier
To construct large-insert libraries for the sequencing, mapping, and functional studies of
complex genomes, we have constructed a new modular bacterial artificial chromosome …

An anchored framework BAC map of mouse chromosome 11 assembled using multiplex oligonucleotide hybridization

WW Cai, J Reneker, CW Chow, M Vaishnav, A Bradley - Genomics, 1998 - Elsevier
Despite abundant library resources for many organisms, physical mapping of these
organisms has been seriously limited due to lack of efficient library screening techniques …

A bacterial artificial chromosome library for sequencing the complete human genome

K Osoegawa, AG Mammoser, C Wu, E Frengen… - Genome …, 2001 - genome.cshlp.org
A 30-fold redundant human bacterial artificial chromosome (BAC) library with a large
average insert size (178 kb) has been constructed to provide the intermediate substrate for …

Construction and characterization of an eightfold redundant dog genomic bacterial artificial chromosome library

R Li, E Mignot, J Faraco, H Kadotani, J Cantanese… - Genomics, 1999 - Elsevier
A large insert canine genomic bacterial artificial chromosome (BAC) library was built from a
Doberman pinscher. Approximately 166,000 clones were gridded on nine high-density …

Construction and characterization of a human bacterial artificial chromosome library

UJ Kim, BW Birren, T Slepak, V Mancino, C Boysen… - Genomics, 1996 - Elsevier
We have constructed an arrayed human genomic BAC library with approximately 4×
coverage that is represented by 96,000 BAC clones with average insert size of nearly 140 …

High throughput direct end sequencing of BAC clones

JM Kelley, CE Field, M Brook Craven… - Nucleic Acids …, 1999 - academic.oup.com
Libraries constructed in bacterial artificial chromosome (BAC) vectors have become the
choice for clone sets in high throughput genomic sequencing projects primarily because of …

YACs, BACs, PACs and MACs: artificial chromosomes as research tools

AP Monaco, Z Larin - Trends in biotechnology, 1994 - cell.com
Artificial chromosomes have now become a major research tool in both genome analysis
and in the functional characterization of genes. Yeast artificial chromosomes (YACs)(R. ef. 1; …

Human chromosome–specific DNA libraries: Construction and availability

MA Van Dilla, LL Deaven, KL Albright, NA Allen… - Bio/technology, 1986 - nature.com
The goal of the National Laboratory Gene Library Project at the Los Alamos and Lawrence
Livermore National Laboratories is the production of chromosome–specific human gene …