[PDF][PDF] Improving protein production on the level of regulation of both expression and secretion pathways in Bacillus subtilis

Y Song, JM Nikoloff, D Zhan - Journal of microbiology and …, 2015 - koreascience.kr
The well-characterized gram-positive bacterium Bacillus subtilis is an outstanding industrial
candidate for protein expression owing to its single membrane and high capacity of …

Protein secretion pathways in Bacillus subtilis: implication for optimization of heterologous protein secretion

LL Fu, ZR Xu, WF Li, JB Shuai, P Lu, CX Hu - Biotechnology advances, 2007 - Elsevier
The absence of an outer membrane in Bacillus subtilis can simplify the protein secretion
pathways and allow the organism to secrete high levels of extracellular proteins. Of the three …

Construction of protein overproducer strains in Bacillus subtilis by an integrative approach

J Jan, F Valle, F Bolivar, E Merino - Applied microbiology and …, 2001 - Springer
We evaluated the effect of several genetic factors reported as having a role in the induction
of the expression of significant levels of recombinant protein in Bacillus subtilis. We utilized …

An overview and future prospects of recombinant protein production in Bacillus subtilis

H Yang, J Qu, W Zou, W Shen, X Chen - Applied Microbiology and …, 2021 - Springer
Bacillus subtilis is a well-characterized Gram-positive bacterium and a valuable host for
recombinant protein production because of its efficient secretion ability, high yield, and non …

The multifunctionality of expression systems in Bacillus subtilis: Emerging devices for the production of recombinant proteins

CC Souza, JM Guimarães, SS Pereira… - Experimental …, 2021 - journals.sagepub.com
Bacillus subtilis is a successful host for producing recombinant proteins. Its GRAS (generally
recognized as safe) status and its remarkable innate ability to absorb and incorporate …

The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat-or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via …

MAB Kolkman, R van der Ploeg, M Bertels… - Applied and …, 2008 - Am Soc Microbiol
Proteins that are produced for commercial purposes in Bacillus subtilis are commonly
secreted via the Sec pathway. Despite its high secretion capacity, the secretion of …

Optimization of protein secretion by Bacillus subtilis

R Nijland, OP Kuipers - Recent Patents on Biotechnology, 2008 - ingentaconnect.com
The gram-positive bacterium Bacillus subtilis is widely known for its capacity to produce and
secrete large amounts of industrially relevant proteins, mostly endogenous enzymes like …

A genetic toolkit for efficient production of secretory protein in Bacillus subtilis

Y Li, Y Wu, Y Liu, J Li, G Du, X Lv, L Liu - Bioresource Technology, 2022 - Elsevier
Bacillus subtilis is a microbial cell factory widely used to produce recombinant proteins, but
the expression of heterologous proteins is often severely hampered. This study constructed …

Exploitation of Bacillus subtilis as a robust workhorse for production of heterologous proteins and beyond

W Cui, L Han, F Suo, Z Liu, L Zhou, Z Zhou - World Journal of Microbiology …, 2018 - Springer
Bacillus subtilis, belonging to the type species of Bacillus, is a type of soil-derived, low% G+
C, endospore-forming Gram-positive bacterium. After the discovery of B. subtilis 168 that …

[HTML][HTML] Bacillus subtilis as cell factory for pharmaceutical proteins: a biotechnological approach to optimize the host organism

L Westers, H Westers, WJ Quax - … et Biophysica Acta (BBA)-Molecular Cell …, 2004 - Elsevier
Bacillus subtilis is a rod-shaped, Gram-positive soil bacterium that secretes numerous
enzymes to degrade a variety of substrates, enabling the bacterium to survive in a …