Abstract The Tn 5-based transposon Tn 5-KIL3 (Miksch et al. 1997c) bearing the kil gene of
the ColE1 plasmid of Escherichia coli, which mediates controlled export of periplasmic …

A Tn 5-based transposon bearing the kil gene (killing protein), mediating controlled export of
periplasmic proteins into the culture medium, was constructed (Tn 5-KIL3). This transposon …

Heterologous gene products produced by Escherichia coli cells can be exported into the
culture medium by the action of the kil gene of the ColE1 plasmid, which encodes a bacterial …

The extracellular production of a hybrid bacterial β-glucanase using Escherichia coli was
studied by using combinations of promoters of varying strength for both a β-glucanase as the …

Cultivation conditions for the extracellular production of a hybrid β-glucanase from Bacillus
were established by using Escherichiacoli JM109 carrying the plasmid pLF3. This plasmid …

DNA fragments containing the Klebsiella oxytoca genes encoding β-glucosidase and
amylase were cloned into the kanamycin resistance transposon Tn 5. Another DNA fragment …

By using a β-glucanase from Bacillus as a model protein, we investigated whether the
secretion competence based on the action of the kil gene can be improved using stronger …

A cartridge was constructed which contained the divergent tet promoters of transposon Tn
10 between an exoglucanase gene (cex) and an endoglucanase gene (cenA) of …

Microorganisms are widely used in industrial biotechnology as cell factories for the
sustainable production of a wide range of compounds and chemicals. For example, virtually …

The emergence of recombinant DNA technology during the early 70's set a revolution in
molecular biology. This set of techniques was strengthened even further later on with the …